C57BL/6NCya-Tcn2em1flox/Cya
Common Name:
Tcn2-flox
Product ID:
S-CKO-05844
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Tcn2-flox
Strain ID
CKOCMP-21452-Tcn2-B6N-VA
Gene Name
Product ID
S-CKO-05844
Gene Alias
Tcn-2
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Tcn2em1flox/Cya mice (Catalog S-CKO-05844) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109993
NCBI RefSeq
NM_001130458
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Tcn2, also known as transcobalamin-2, is an essential plasma protein crucial for the absorption, transportation, and cellular uptake of vitamin B12 [3]. It is involved in one-carbon metabolism pathways, which are vital for various biological processes such as DNA synthesis, methylation reactions, and cell proliferation [2].
In zebrafish, loss-of-function studies of tcn2 (the zebrafish ortholog of human Tcn2) showed that homozygous tcn2 -/- fish have reduced growth, and offspring of tcn2 -/- females exhibit gross developmental and metabolic defects, which can be rescued by vitamin B-12 supplementation, suggesting that Tcn2-mediated vitamin B-12 deposition in the yolk is necessary for early development [4]. In a mouse model relevant to multiple sclerosis, Tcn2 functions as a chaperone for FTY720, and the B12-Tcn2-CD320 pathway is essential for FTY720's mechanism of action in suppressing neuroinflammation [1].
In conclusion, Tcn2 is of great biological importance for vitamin B12-related processes. Studies using gene-knockout models in zebrafish and mice have revealed its roles in development and in diseases like multiple sclerosis, highlighting its potential as a therapeutic target in related conditions.
References：
1. Jonnalagadda, Deepa, Kihara, Yasuyuki, Groves, Aran, Rivera, Richard, Chun, Jerold. 2023. FTY720 requires vitamin B12-TCN2-CD320 signaling in astrocytes to reduce disease in an animal model of multiple sclerosis. In Cell reports, 42, 113545. doi:10.1016/j.celrep.2023.113545. https://pubmed.ncbi.nlm.nih.gov/38064339/
2. Oussalah, Abderrahim, Levy, Julien, Filhine-Trésarrieu, Pierre, Namour, Fares, Guéant, Jean-Louis. 2017. Association of TCN2 rs1801198 c.776G>C polymorphism with markers of one-carbon metabolism and related diseases: a systematic review and meta-analysis of genetic association studies. In The American journal of clinical nutrition, 106, 1142-1156. doi:10.3945/ajcn.117.156349. https://pubmed.ncbi.nlm.nih.gov/28814397/
3. Kose, Engin, Besci, Ozge, Gudeloglu, Elif, Ozen, Selime, Isguder, Rana. . Transcobalamin II deficiency in twins with a novel variant in the TCN2 gene: case report and review of literature. In Journal of pediatric endocrinology & metabolism : JPEM, 33, 1487-1499. doi:10.1515/jpem-2020-0096. https://pubmed.ncbi.nlm.nih.gov/32841161/
4. Benoit, Courtney R, Walsh, Darren J, Mekerishvili, Levan, McGaughey, David M, Brody, Lawrence C. . Loss of the Vitamin B-12 Transport Protein Tcn2 Results in Maternally Inherited Growth and Developmental Defects in Zebrafish. In The Journal of nutrition, 151, 2522-2532. doi:10.1093/jn/nxab151. https://pubmed.ncbi.nlm.nih.gov/34132337/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen