C57BL/6JCya-Fam20bem1flox/Cya
Common Name:
Fam20b-flox
Product ID:
S-CKO-05886
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fam20b-flox
Strain ID
CKOCMP-215015-Fam20b-B6J-VA
Gene Name
Product ID
S-CKO-05886
Gene Alias
C530043G21Rik; mKIAA0475
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fam20bem1flox/Cya mice (Catalog S-CKO-05886) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086153
NCBI RefSeq
NM_145413
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Fam20b, a protein-coding gene, is a newly identified xylose kinase. It phosphorylates xylose in the glycosaminoglycan-protein linkage region, which is essential for glycosaminoglycan (GAG) chain elongation and assembly [3]. GAGs are crucial components of the extracellular matrix (ECM), and thus, Fam20b is implicated in ECM-related biological processes.
In gene-knockout studies, inactivating Fam20b in the dental epithelium of mice led to supernumerary tooth formation. This occurs as Fam20B-catalyzed GAGs regulate Sox2⁺ dental epithelial stem/progenitor cell homeostasis by restricting FGFR2b signaling at the initial tooth development stage [1,6]. In Wnt1-Cre; Fam20bf/f mice, where Fam20b was inactivated in neural crest-derived mesenchyme, complete cleft palate, malformed tongue, and micrognathia were observed due to reduced GAG chains promoting palatal cell apoptosis and impaired osteogenesis [2,5]. Also, in Fam20B conditional knockout mice with inactivation in type I collagen-expressing cells, severe spine deformity and intervertebral disc defects were seen, along with cell fate changes in the annulus fibrosus via alterations in TGF-β and MAPK signaling pathways [4].
In conclusion, Fam20b is essential for various developmental processes. Its function in GAG chain synthesis impacts tissue morphogenesis and homeostasis. The Fam20b KO/CKO mouse models have significantly contributed to understanding its role in craniofacial development, tooth formation, and intervertebral disc development, providing insights into related congenital defects and disorders [1-2, 5, 7, 9].
References:
1. Wu, Jingyi, Tian, Ye, Han, Lu, Linhardt, Robert J, Wang, Xiaofang. 2020. FAM20B-catalyzed glycosaminoglycans control murine tooth number by restricting FGFR2b signaling. In BMC biology, 18, 87. doi:10.1186/s12915-020-00813-4. https://pubmed.ncbi.nlm.nih.gov/32664967/
2. Chen, Xiaoyan, Li, Nan, Hu, Ping, Xiao, Jing, Liu, Chao. 2023. Deficiency of Fam20b-Catalyzed Glycosaminoglycan Chain Synthesis in Neural Crest Leads to Cleft Palate. In International journal of molecular sciences, 24, . doi:10.3390/ijms24119634. https://pubmed.ncbi.nlm.nih.gov/37298583/
3. Koike, Toshiyasu, Izumikawa, Tomomi, Tamura, Jun-Ichi, Kitagawa, Hiroshi. 2009. FAM20B is a kinase that phosphorylates xylose in the glycosaminoglycan-protein linkage region. In The Biochemical journal, 421, 157-62. doi:10.1042/BJ20090474. https://pubmed.ncbi.nlm.nih.gov/19473117/
4. Saiyin, Wuliji, Li, Lili, Zhang, Hua, Lu, Yongbo, Qin, Chunlin. 2019. Inactivation of FAM20B causes cell fate changes in annulus fibrosus of mouse intervertebral disc and disc defects via the alterations of TGF-β and MAPK signaling pathways. In Biochimica et biophysica acta. Molecular basis of disease, 1865, 165555. doi:10.1016/j.bbadis.2019.165555. https://pubmed.ncbi.nlm.nih.gov/31513834/
5. Liu, Xuena, Li, Nan, Zhang, Hua, Xiao, Jing, Liu, Chao. 2018. Inactivation of Fam20b in the neural crest-derived mesenchyme of mouse causes multiple craniofacial defects. In European journal of oral sciences, 126, 433-436. doi:10.1111/eos.12563. https://pubmed.ncbi.nlm.nih.gov/30105814/
6. Tian, Ye, Ma, Pan, Liu, Chao, Qin, Chunlin, Wang, Xiaofang. 2015. Inactivation of Fam20B in the dental epithelium of mice leads to supernumerary incisors. In European journal of oral sciences, 123, 396-402. doi:10.1111/eos.12222. https://pubmed.ncbi.nlm.nih.gov/26465965/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen