C57BL/6JCya-Miga1em1flox/Cya
Common Name:
Miga1-flox
Product ID:
S-CKO-05933
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Miga1-flox
Strain ID
CKOCMP-215708-Miga1-B6J-VA
Gene Name
Product ID
S-CKO-05933
Gene Alias
A930038D06; C030011O14Rik; Fam73a; Mita1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Miga1em1flox/Cya mice (Catalog S-CKO-05933) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000073089
NCBI RefSeq
NM_174868
Target Region
Exon 3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Miga1, a mammalian homolog of the mitoguardin gene, encodes a mitochondrial outer membrane protein. It is crucial for mitochondrial fusion, a process that maintains mitochondrial morphology and function. Miga1 is involved in pathways such as the Hippo-YAP1 signaling pathway and may collaborate with protein kinase B (AKT) [1]. It also plays a role in processes related to cell metabolism and is important for normal cellular functions. Genetic models, like knockout mouse models, are valuable tools for studying Miga1's function.
In Miga1 knockout mouse models, female mice were found to be subfertile. The ovarian phenotypes indicated that Miga1 plays an important role in ovulation and ovarian steroidogenesis. In Miga1-knockout granulosa cells, there were severe defects in luteinization and steroidogenesis, along with disordered mitochondrial morphology and function in response to gonadotropins [3]. In differentiated adipocytes, down-regulation of Miga1 led to mitochondrial dysfunction, with lower oxygen consumption and decreased UCP1 expression, suggesting its role in adipocyte function and potentially fat distribution [2,4].
In conclusion, Miga1 is essential for maintaining mitochondrial function, which is crucial for processes like ovarian endocrine functions and adipocyte function. The use of Miga1 knockout mouse models has revealed its significance in subfertility-related issues and potentially in diseases related to abnormal fat distribution. These findings contribute to our understanding of the molecular pathogenesis of reproductive endocrine diseases and metabolic disorders.
References:
1. Yan, Ming-Qi, Zhu, Bing-Hong, Liu, Xiao-Hong, Li, Tao, Liu, Xiao-Man. 2023. Mitoguardin 1 and 2 promote granulosa cell proliferation by activating AKT and regulating the Hippo-YAP1 signaling pathway. In Cell death & disease, 14, 779. doi:10.1038/s41419-023-06312-y. https://pubmed.ncbi.nlm.nih.gov/38012141/
2. Reed, Jordan N, Huang, Jiansheng, Li, Yong, Björkegren, Johan Lm, Civelek, Mete. 2024. Systems genetics analysis of human body fat distribution genes identifies adipocyte processes. In Life science alliance, 7, . doi:10.26508/lsa.202402603. https://pubmed.ncbi.nlm.nih.gov/38702075/
3. Liu, Xiao-Man, Zhang, Yin-Li, Ji, Shu-Yan, Tong, Chao, Fan, Heng-Yu. . Mitochondrial Function Regulated by Mitoguardin-1/2 Is Crucial for Ovarian Endocrine Functions and Ovulation. In Endocrinology, 158, 3988-3999. doi:10.1210/en.2017-00487. https://pubmed.ncbi.nlm.nih.gov/28938432/
4. Reed, Jordan N, Huang, Jiansheng, Li, Yong, Björkegren, Johan L M, Civelek, Mete. 2023. Systems genetics analysis of human body fat distribution genes identifies Wnt signaling and mitochondrial activity in adipocytes. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.09.06.556534. https://pubmed.ncbi.nlm.nih.gov/37732278/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen