C57BL/6JCya-Tcp11l2em1flox/Cya
Common Name
Tcp11l2-flox
Product ID
S-CKO-05977
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-216198-Tcp11l2-B6J-VA
Status
When using this mouse strain in a publication, please cite “Tcp11l2-flox Mouse (Catalog S-CKO-05977) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Tcp11l2-flox
Strain ID
CKOCMP-216198-Tcp11l2-B6J-VA
Gene Name
Product ID
S-CKO-05977
Gene Alias
E430026E19Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000020223
NCBI RefSeq
NM_146008
Target Region
Exon 3
Size of Effective Region
~1.9 kb
Overview of Gene Research
Tcp11l2, T-complex 11 like 2, is a protein-coding gene. It contains a serine-rich region in its N-terminal region. Although its full-scope function was unclear previously, studies have shown its importance in muscle-related biological processes.
In bovine skeletal muscle-derived satellite cells (MDSCs), TCP11L2 expression gradually increases during differentiation in vitro, peaking on Day 3, which is the migration and fusion stage. Using CRISPR/dCas9 gene-editing technology to modulate its expression, it was found that TCP11L2 promotes MDSC differentiation. Wound-healing assays demonstrated its role in promoting MDSC migration during differentiation. It is mainly distributed around microfilaments and microtubules, affects the expression of the actin-related protein 2/3 (ARP2/3) complex, and interacts with formin-like 2 (FMNL2). FMNL2 affects the expression of ARP2/3 to promote the migration of bovine MDSCs, and when FMNL2 is inhibited, the activities of TCP11L2 during MDSC differentiation and migration are blocked [1].
In conclusion, TCP11L2 is crucial for promoting the migration and differentiation of bovine skeletal muscle-derived satellite cells through its interaction with FMNL2. This research on TCP11L2 in the context of muscle-derived satellite cells provides insights into muscle development and related biological processes, potentially contributing to understanding muscle-related diseases or muscle-regeneration strategies.
References：
1. Li, Shuang, Wang, Zhiqi, Tong, Huili, Li, Shufeng, Yan, Yunqin. 2020. TCP11L2 promotes bovine skeletal muscle-derived satellite cell migration and differentiation via FMNL2. In Journal of cellular physiology, 235, 7183-7193. doi:10.1002/jcp.29617. https://pubmed.ncbi.nlm.nih.gov/32017087/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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