Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Tph2em1flox/Cya
Common Name:
Tph2-flox
Product ID:
S-CKO-05985
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tph2-flox
Strain ID
CKOCMP-216343-Tph2-B6J-VA
Gene Name
Tph2
Product ID
S-CKO-05985
Gene Alias
Ntph
Background
C57BL/6JCya
NCBI ID
216343
Modification
Conditional knockout
Chromosome
10
Phenotype
MGI:2651811
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tph2em1flox/Cya mice (Catalog S-CKO-05985) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006949
NCBI RefSeq
NM_173391
Target Region
Exon 5
Size of Effective Region
~1.0 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Tph2, also known as tryptophan hydroxylase 2, is the rate-limiting enzyme in brain serotonin synthesis. Serotonin is involved in numerous physiological, behavioral, and cognitive processes, and Tph2's role in its synthesis makes it biologically significant. Animal models generated by genetic manipulation of Tph2 have been crucial in understanding serotonergic neurotransmission [4].

In Tph2-deficient mice (Tph2 -/-), ethanol consumption increased, suggesting central 5-HT, synthesized by Tph2, modulates drinking behavior [1]. Adult-onset TPH2 deletion in the dorsal Raphe nuclei (DRN) of male mice led to hyperphagia and weight gain, while in females, it affected responses to estrogen-related appetite regulation, indicating a sex-dependent role in energy balance [2]. Also, deletion of TLR4 in Tph2 neurons decreased anxiety-like behaviors in male mice, showing a sex-specific modulation of anxiety-related behaviors [3]. In TPH2-R439H mice (a model with a mutation analogous to that in severely depressed individuals), there were defects in enteric nervous system (ENS) development and gastrointestinal (GI) motility due to reduced 5-HT release from enteric neurons, and Tph2 CKO in APP/PS1 mice increased amyloid plaque load and astrogliosis [5,6].

In conclusion, Tph2 plays essential roles in multiple biological processes. Through gene-knockout and related mouse models, it has been shown to be involved in alcohol-related behaviors, energy balance regulation, anxiety-related behaviors, ENS development, GI motility, and Alzheimer's-related amyloid plaque formation. These findings contribute to understanding the underlying mechanisms of related diseases and biological functions [1,2,3,5,6].

References:
1. Zaniewska, Magdalena, Mosienko, Valentina, Bader, Michael, Alenina, Natalia. 2022. Tph2 Gene Expression Defines Ethanol Drinking Behavior in Mice. In Cells, 11, . doi:10.3390/cells11050874. https://pubmed.ncbi.nlm.nih.gov/35269497/
2. Liu, Hailan, Wang, Chunmei, Yu, Meng, Hu, Fang, Xu, Yong. . TPH2 in the Dorsal Raphe Nuclei Regulates Energy Balance in a Sex-Dependent Manner. In Endocrinology, 162, . doi:10.1210/endocr/bqaa183. https://pubmed.ncbi.nlm.nih.gov/33034617/
3. Li, Yongxiang, Zhu, Shuqing, Xie, Kailai, Jiang, Qingyan, Wang, Lina. 2022. TLR4 in Tph2 neurons modulates anxiety-related behaviors in a sex-dependent manner. In Neuropharmacology, 216, 109175. doi:10.1016/j.neuropharm.2022.109175. https://pubmed.ncbi.nlm.nih.gov/35787402/
4. Pratelli, Marta, Pasqualetti, Massimo. 2018. Serotonergic neurotransmission manipulation for the understanding of brain development and function: Learning from Tph2 genetic models. In Biochimie, 161, 3-14. doi:10.1016/j.biochi.2018.11.016. https://pubmed.ncbi.nlm.nih.gov/30513372/
5. Israelyan, Narek, Del Colle, Andrew, Li, Zhishan, Caron, Marc G, Margolis, Kara G. 2019. Effects of Serotonin and Slow-Release 5-Hydroxytryptophan on Gastrointestinal Motility in a Mouse Model of Depression. In Gastroenterology, 157, 507-521.e4. doi:10.1053/j.gastro.2019.04.022. https://pubmed.ncbi.nlm.nih.gov/31071306/
6. Xu, Chao-Jin, Wang, Jun-Ling. . Tph2 Genetic Ablation Contributes to Senile Plaque Load and Astrogliosis in APP/PS1 Mice. In Current Alzheimer research, 16, 219-232. doi:10.2174/1567205016666190301110110. https://pubmed.ncbi.nlm.nih.gov/30827242/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest