C57BL/6JCya-Tph2em1flox/Cya
Common Name:
Tph2-flox
Product ID:
S-CKO-05985
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tph2-flox
Strain ID
CKOCMP-216343-Tph2-B6J-VA
Gene Name
Product ID
S-CKO-05985
Gene Alias
Ntph
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tph2em1flox/Cya mice (Catalog S-CKO-05985) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006949
NCBI RefSeq
NM_173391
Target Region
Exon 5
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Tph2, also known as tryptophan hydroxylase 2, is the rate-limiting enzyme in brain serotonin synthesis. Serotonin is involved in numerous physiological, behavioral, and cognitive processes, and Tph2's role in its synthesis makes it biologically significant. Animal models generated by genetic manipulation of Tph2 have been crucial in understanding serotonergic neurotransmission [4].
In Tph2-deficient mice (Tph2 -/-), ethanol consumption increased, suggesting central 5-HT, synthesized by Tph2, modulates drinking behavior [1]. Adult-onset TPH2 deletion in the dorsal Raphe nuclei (DRN) of male mice led to hyperphagia and weight gain, while in females, it affected responses to estrogen-related appetite regulation, indicating a sex-dependent role in energy balance [2]. Also, deletion of TLR4 in Tph2 neurons decreased anxiety-like behaviors in male mice, showing a sex-specific modulation of anxiety-related behaviors [3]. In TPH2-R439H mice (a model with a mutation analogous to that in severely depressed individuals), there were defects in enteric nervous system (ENS) development and gastrointestinal (GI) motility due to reduced 5-HT release from enteric neurons, and Tph2 CKO in APP/PS1 mice increased amyloid plaque load and astrogliosis [5,6].
In conclusion, Tph2 plays essential roles in multiple biological processes. Through gene-knockout and related mouse models, it has been shown to be involved in alcohol-related behaviors, energy balance regulation, anxiety-related behaviors, ENS development, GI motility, and Alzheimer's-related amyloid plaque formation. These findings contribute to understanding the underlying mechanisms of related diseases and biological functions [1,2,3,5,6].
References:
1. Zaniewska, Magdalena, Mosienko, Valentina, Bader, Michael, Alenina, Natalia. 2022. Tph2 Gene Expression Defines Ethanol Drinking Behavior in Mice. In Cells, 11, . doi:10.3390/cells11050874. https://pubmed.ncbi.nlm.nih.gov/35269497/
2. Liu, Hailan, Wang, Chunmei, Yu, Meng, Hu, Fang, Xu, Yong. . TPH2 in the Dorsal Raphe Nuclei Regulates Energy Balance in a Sex-Dependent Manner. In Endocrinology, 162, . doi:10.1210/endocr/bqaa183. https://pubmed.ncbi.nlm.nih.gov/33034617/
3. Li, Yongxiang, Zhu, Shuqing, Xie, Kailai, Jiang, Qingyan, Wang, Lina. 2022. TLR4 in Tph2 neurons modulates anxiety-related behaviors in a sex-dependent manner. In Neuropharmacology, 216, 109175. doi:10.1016/j.neuropharm.2022.109175. https://pubmed.ncbi.nlm.nih.gov/35787402/
4. Pratelli, Marta, Pasqualetti, Massimo. 2018. Serotonergic neurotransmission manipulation for the understanding of brain development and function: Learning from Tph2 genetic models. In Biochimie, 161, 3-14. doi:10.1016/j.biochi.2018.11.016. https://pubmed.ncbi.nlm.nih.gov/30513372/
5. Israelyan, Narek, Del Colle, Andrew, Li, Zhishan, Caron, Marc G, Margolis, Kara G. 2019. Effects of Serotonin and Slow-Release 5-Hydroxytryptophan on Gastrointestinal Motility in a Mouse Model of Depression. In Gastroenterology, 157, 507-521.e4. doi:10.1053/j.gastro.2019.04.022. https://pubmed.ncbi.nlm.nih.gov/31071306/
6. Xu, Chao-Jin, Wang, Jun-Ling. . Tph2 Genetic Ablation Contributes to Senile Plaque Load and Astrogliosis in APP/PS1 Mice. In Current Alzheimer research, 16, 219-232. doi:10.2174/1567205016666190301110110. https://pubmed.ncbi.nlm.nih.gov/30827242/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen