C57BL/6JCya-Agap2em1flox/Cya
Common Name:
Agap2-flox
Product ID:
S-CKO-05992
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Agap2-flox
Strain ID
CKOCMP-216439-Agap2-B6J-VA
Gene Name
Product ID
S-CKO-05992
Gene Alias
AGAP-2; Centg1; PIKE; cnt-g1; mKIAA0167
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Agap2em1flox/Cya mice (Catalog S-CKO-05992) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000039259
NCBI RefSeq
NM_001033263
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
AGAP2, also known as Arf GAP with GTP-binding protein-like domain, Ankyrin repeat and PH domain 2, is a protein belonging to the Arf GAP (GTPase activating protein) protein family. These proteins act as GTPase switches for Arfs (Ras superfamily members), thus being involved in signaling regulation. AGAP2 has GTPase activity and participates in multiple cellular functions like membrane trafficking, actin cytoskeleton remodeling, apoptosis, cell survival, migration, and receptor trafficking [1].
AGAP2's increase is associated with pathologies such as cancer and fibrosis. In liver fibrosis, its expression modulates some of the pro-fibrotic effects of Transforming growth factor beta-1 (TGF-β1) [1]. In clear cell renal cell carcinoma (ccRCC), AGAP2 is upregulated compared to normal tissues, and its higher expression is related to clinical cancer stages, TNM stages, pathologic stages, and status. Prognostic analysis shows that its overexpression is associated with reduced overall survival in KIRC, while it may improve the OS in some other cancers. GO and KEGG analysis indicate AGAP2-related genes are linked to T cell activation, immune activity, and PD-L1 expression and PD-1 checkpoint pathway, and AGAP2 is significantly associated with multiple types of immune cells and affects immune cell infiltration levels [3]. In phagocytes, AGAP2 localizes at actin-rich phagocytic cups and increases Fcγ receptor-mediated phagocytosis. The GAP domain of AGAP2, but not its GAP activity, is important in FcγR-dependent uptake of opsonized particles [2].
In conclusion, AGAP2 is a multi-functional protein involved in various cellular signaling pathways and biological functions. Its dysregulation is associated with diseases like cancer and fibrosis. Studies on AGAP2, especially through gene-based models in different disease contexts, help in understanding disease mechanisms and could potentially lead to new therapeutic strategies for these diseases.
References:
1. Navarro-Corcuera, Amaia, Ansorena, Eduardo, Montiel-Duarte, Cristina, Iraburu, María J. 2020. AGAP2: Modulating TGFβ1-Signaling in the Regulation of Liver Fibrosis. In International journal of molecular sciences, 21, . doi:10.3390/ijms21041400. https://pubmed.ncbi.nlm.nih.gov/32092977/
2. Chouinard, François C, Davis, Lynn, Gilbert, Caroline, Bourgoin, Sylvain G. 2022. Functional Role of AGAP2/PIKE-A in Fcγ Receptor-Mediated Phagocytosis. In Cells, 12, . doi:10.3390/cells12010072. https://pubmed.ncbi.nlm.nih.gov/36611866/
3. Xu, Zekun, Wang, Yuxuan, Xu, Jiangnan, Xu, Min, Pei, Changsong. 2023. Identify AGAP2 as prognostic biomarker in clear cell renal cell carcinoma based on bioinformatics and IHC staining. In Heliyon, 9, e13543. doi:10.1016/j.heliyon.2023.e13543. https://pubmed.ncbi.nlm.nih.gov/36846683/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen