C57BL/6JCya-Tead1em1flox/Cya
Common Name:
Tead1-flox
Product ID:
S-CKO-06031
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tead1-flox
Strain ID
CKOCMP-21676-Tead1-B6J-VA
Gene Name
Product ID
S-CKO-06031
Gene Alias
2610024B07Rik; B230114H05Rik; Gtrgeo5; TEAD-1; TEF-1; Tcf13; mTEF-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tead1em1flox/Cya mice (Catalog S-CKO-06031) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059768
NCBI RefSeq
NM_001166584
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
TEAD1, or TEA domain transcription factor 1, is a crucial transcription factor in the Hippo pathway, which regulates cellular proliferation, organ size, and tissue homeostasis [1,2,3,4,5,6,7,8,9]. It plays a vital role in multiple biological processes including heart development, homeostasis, and cell survival, and is associated with various diseases such as liver fibrosis, heart failure, and dilated cardiomyopathy [1,2,3,4]. Genetic models, like gene knockout (KO) and conditional knockout (CKO) mouse models, have been instrumental in studying its functions.
In liver, deletion of TM7SF3 accelerates hepatic stellate cell (HSC) activation, promoting alternative splicing of TEAD1 and generating a more active form, which triggers HSC activation and liver fibrosis. Inhibiting TEAD1 alternative splicing can reduce liver fibrosis [1]. In the heart, conditional TEAD1 knockout in cardiac fibroblasts and myofibroblasts ameliorates pressure overload-induced cardiac remodeling [2]. Ubiquitous or cardiomyocyte-specific Tead1 loss in adult mice leads to acute-onset dilated cardiomyopathy due to activation of the necroptotic pathway in cardiomyocytes [6]. Also, perinatal cardiomyocyte-specific deletion of Tead1 causes lethal dilated cardiomyopathy by postnatal day 9, as Tead1-deficient cardiomyocytes have decreased proliferation [9].
In conclusion, TEAD1 is essential for normal cell proliferation, especially in cardiomyocytes during the perinatal period, and its dysregulation contributes to the development of liver fibrosis and various heart diseases. Studies using KO and CKO mouse models have significantly enhanced our understanding of TEAD1's functions in these disease-related biological processes, providing potential therapeutic targets for treatment.
References:
1. Isaac, Roi, Bandyopadhyay, Gautam, Rohm, Theresa V, Webster, Nicholas J G, Olefsky, Jerrold M. 2024. TM7SF3 controls TEAD1 splicing to prevent MASH-induced liver fibrosis. In Cell metabolism, 36, 1030-1043.e7. doi:10.1016/j.cmet.2024.04.003. https://pubmed.ncbi.nlm.nih.gov/38670107/
2. Song, Shuai, Zhang, Xiaokai, Huang, Zihang, Sun, Aijun, Ge, Junbo. 2024. TEA domain transcription factor 1(TEAD1) induces cardiac fibroblasts cells remodeling through BRD4/Wnt4 pathway. In Signal transduction and targeted therapy, 9, 45. doi:10.1038/s41392-023-01732-w. https://pubmed.ncbi.nlm.nih.gov/38374140/
3. Shi, Xin, Dang, Xuening, Huang, Zhenyu, Gu, Chang, He, Ben. 2024. SUMOylation of TEAD1 Modulates the Mechanism of Pathological Cardiac Hypertrophy. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2305677. doi:10.1002/advs.202305677. https://pubmed.ncbi.nlm.nih.gov/38225750/
4. Yamada, Shintaro, Ko, Toshiyuki, Ito, Masamichi, Aburatani, Hiroyuki, Komuro, Issei. 2023. TEAD1 trapping by the Q353R-Lamin A/C causes dilated cardiomyopathy. In Science advances, 9, eade7047. doi:10.1126/sciadv.ade7047. https://pubmed.ncbi.nlm.nih.gov/37058558/
5. Zhang, Sitong, Sun, Zhongquan, Chen, Zhenhua, Ding, Yuan, Wang, Weilin. 2024. Endothelial YAP/TEAD1-CXCL17 signaling recruits myeloid-derived suppressor cells against liver ischemia-reperfusion injury. In Hepatology (Baltimore, Md.), 81, 888-902. doi:10.1097/HEP.0000000000000773. https://pubmed.ncbi.nlm.nih.gov/38407233/
6. Liu, Jinhua, Wen, Tong, Dong, Kunzhe, Zhang, Wei, Zhou, Jiliang. 2021. TEAD1 protects against necroptosis in postmitotic cardiomyocytes through regulation of nuclear DNA-encoded mitochondrial genes. In Cell death and differentiation, 28, 2045-2059. doi:10.1038/s41418-020-00732-5. https://pubmed.ncbi.nlm.nih.gov/33469230/
7. Li, Feng, Negi, Vinny, Yang, Ping, Moulik, Mousumi, Yechoor, Vijay K. . TEAD1 regulates cell proliferation through a pocket-independent transcription repression mechanism. In Nucleic acids research, 50, 12723-12738. doi:10.1093/nar/gkac1063. https://pubmed.ncbi.nlm.nih.gov/36484096/
8. Shen, Tianyu, Li, Yang, Wang, Dekun, Niu, Yuanjie, Tan, Xiaoyue. 2022. YAP1-TEAD1 mediates the perineural invasion of prostate cancer cells induced by cancer-associated fibroblasts. In Biochimica et biophysica acta. Molecular basis of disease, 1868, 166540. doi:10.1016/j.bbadis.2022.166540. https://pubmed.ncbi.nlm.nih.gov/36100154/
9. Liu, Ruya, Jagannathan, Rajaganapathi, Li, Feng, Yechoor, Vijay K, Moulik, Mousumi. 2019. Tead1 is required for perinatal cardiomyocyte proliferation. In PloS one, 14, e0212017. doi:10.1371/journal.pone.0212017. https://pubmed.ncbi.nlm.nih.gov/30811446/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen