C57BL/6JCya-Kdm6bem1flox/Cya
Common Name:
Kdm6b-flox
Product ID:
S-CKO-06059
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Kdm6b-flox
Strain ID
CKOCMP-216850-Kdm6b-B6J-VA
Gene Name
Product ID
S-CKO-06059
Gene Alias
1700064E03Rik; Jmjd3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kdm6bem1flox/Cya mice (Catalog S-CKO-06059) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000094077
NCBI RefSeq
NM_001017426
Target Region
Exon 14~20
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Kdm6b, also known as JMJD3, is a histone demethylase that specifically removes methyl groups from H3K27me3, allowing re-expression of its target genes. This function is crucial in regulating gene expression and cell fate, playing roles in multiple biological processes such as development, differentiation, and disease-related pathways [4].
In osteosarcoma, Kdm6b is upregulated and promotes lung metastasis by mediating H3K27me3 demethylation of LDHA [1]. In glioblastoma, myeloid-specific deletion of Kdm6b enhances pro-inflammatory pathways and improves survival in tumor-bearing mice [2]. In cementoblasts, knockdown of Kdm6b inhibits HADHA-related fatty acid oxidation and mineralization [3]. In T-cell acute lymphoblastic leukemia (T-ALL), mouse cells deficient for Kdm6b cannot propagate T-ALL, and inactivating Kdm6b in human T-ALL patient cells leads to reduced cell survival [5].
In summary, Kdm6b is essential for regulating gene expression through histone demethylation. Studies using gene-knockout models in mice have revealed its significant roles in cancer metastasis, immune-related diseases, and tissue-specific cell functions such as cementogenesis. These findings suggest Kdm6b as a potential therapeutic target in relevant disease areas.
References:
1. Jiang, Yuhang, Li, Fengfeng, Gao, Bowen, Liu, Sanhong, Shen, Huiyong. 2021. KDM6B-mediated histone demethylation of LDHA promotes lung metastasis of osteosarcoma. In Theranostics, 11, 3868-3881. doi:10.7150/thno.53347. https://pubmed.ncbi.nlm.nih.gov/33664867/
2. Goswami, Sangeeta, Raychaudhuri, Deblina, Singh, Pratishtha, Basu, Sreyashi, Sharma, Padmanee. 2023. Myeloid-specific KDM6B inhibition sensitizes glioblastoma to PD1 blockade. In Nature cancer, 4, 1455-1473. doi:10.1038/s43018-023-00620-0. https://pubmed.ncbi.nlm.nih.gov/37653141/
3. Yang, Z, Wang, H, Xiao, J, Wang, X, Cao, Z. 2024. KDM6B-Mediated HADHA Demethylation/Lactylation Regulates Cementogenesis. In Journal of dental research, 104, 75-85. doi:10.1177/00220345241286460. https://pubmed.ncbi.nlm.nih.gov/39569625/
4. Lagunas-Rangel, Francisco Alejandro. 2021. KDM6B (JMJD3) and its dual role in cancer. In Biochimie, 184, 63-71. doi:10.1016/j.biochi.2021.02.005. https://pubmed.ncbi.nlm.nih.gov/33581195/
5. Issa, Nancy, Bjeije, Hassan, Wilson, Elisabeth R, Spencer, David H, Challen, Grant A. 2023. KDM6B protects T-ALL cells from NOTCH1-induced oncogenic stress. In Leukemia, 37, 728-740. doi:10.1038/s41375-023-01853-9. https://pubmed.ncbi.nlm.nih.gov/36797416/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen