C57BL/6JCya-Spns2em1flox/Cya
Common Name:
Spns2-flox
Product ID:
S-CKO-06073
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Spns2-flox
Strain ID
CKOCMP-216892-Spns2-B6J-VA
Gene Name
Product ID
S-CKO-06073
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Spns2em1flox/Cya mice (Catalog S-CKO-06073) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000045303
NCBI RefSeq
NM_153060
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Spinster homolog 2 (Spns2) is an S1P transporter belonging to the non-ATP-dependent organic ion transporter family. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid that regulates the immune system, angiogenesis, auditory function, and epithelial and endothelial barrier integrity. Spns2 exports S1P to initiate lipid signaling cascades, playing a crucial role in lymphocyte trafficking, immune responses, and various physiological and pathological processes [1,3,4].
Global deletion of Spns2 (Spns2-/-) has been shown to reduce disease severity in several autoimmune disease models. Postnatal knockout of Spns2 (Spns2-Mx1Cre) also led to a significant reduction of pathogenic Th17 cells in the central nervous system, making these mice resistant to multiple sclerosis in experimental autoimmune encephalomyelitis models. These results suggest that lack of Spns2 affects S1P secretion and lymph node vasculatures, but blood vasculature remains normal. In macrophages, Spns2 deficiency enhances glycolysis, leading to increased intracellular lactate production and subsequent pro-inflammatory response. Reinforcing Spns2/S1P signaling can balance the immune response during sepsis [2,5].
In conclusion, Spns2 is essential for S1P-mediated signaling processes. Gene knockout mouse models, such as Spns2-/-and Spns2-Mx1Cre, have revealed its significant role in autoimmune diseases like multiple sclerosis and in sepsis. Understanding Spns2 function provides potential therapeutic targets for treating these and other related diseases.
References:
1. Chen, Hongwen, Ahmed, Shahbaz, Zhao, Hongtu, Li, Xiaochun, Lee, Chia-Hsueh. 2023. Structural and functional insights into Spns2-mediated transport of sphingosine-1-phosphate. In Cell, 186, 2644-2655.e16. doi:10.1016/j.cell.2023.04.028. https://pubmed.ncbi.nlm.nih.gov/37224812/
2. Fang, Chao, Ren, Pan, Bian, Ganlan, Li, Mingkai, Hou, Zheng. 2023. Enhancing Spns2/S1P in macrophages alleviates hyperinflammation and prevents immunosuppression in sepsis. In EMBO reports, 24, e56635. doi:10.15252/embr.202256635. https://pubmed.ncbi.nlm.nih.gov/37358015/
3. Zhu, Xiao, Ren, Kun, Zeng, Yong-Zhi, Zheng, Zhi, Yi, Guang-Hui. 2018. Biological function of SPNS2: From zebrafish to human. In Molecular immunology, 103, 55-62. doi:10.1016/j.molimm.2018.08.025. https://pubmed.ncbi.nlm.nih.gov/30196234/
4. Spiegel, Sarah, Maczis, Melissa A, Maceyka, Michael, Milstien, Sheldon. 2019. New insights into functions of the sphingosine-1-phosphate transporter SPNS2. In Journal of lipid research, 60, 484-489. doi:10.1194/jlr.S091959. https://pubmed.ncbi.nlm.nih.gov/30655317/
5. Hasan, Zafrul, Nguyen, Toan Q, Lam, Brenda Wan Shing, Angeli, Veronique, Nguyen, Long N. 2022. Postnatal deletion of Spns2 prevents neuroinflammation without compromising blood vascular functions. In Cellular and molecular life sciences : CMLS, 79, 541. doi:10.1007/s00018-022-04573-y. https://pubmed.ncbi.nlm.nih.gov/36198832/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen