C57BL/6JCya-Nagsem1flox/Cya
Common Name:
Nags-flox
Product ID:
S-CKO-06112
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Nags-flox
Strain ID
CKOCMP-217214-Nags-B6J-VA
Gene Name
Product ID
S-CKO-06112
Gene Alias
1700120E20Rik; argA
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nagsem1flox/Cya mice (Catalog S-CKO-06112) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000055409
NCBI RefSeq
NM_145829
Target Region
Exon 2~6
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
NAGS, or N-acetylglutamate synthase, is a mitochondrial enzyme (E.C. 2.3.1.1) [2]. It catalyzes the formation of N-acetylglutamate (NAG), an essential allosteric activator of carbamylphosphate synthetase I (CPSI) in the urea cycle [2]. In microorganisms and plants, it also functions in the arginine biosynthetic pathway [3].
In tumor cells, median expression of NAGS mRNA was higher in glioblastoma multiforme (GBM), glioma, and stomach adenocarcinoma (STAD) samples compared to the matched normal tissue, while in lung adenocarcinoma (LUAD), its expression did not differ from normal tissue. High NAGS expression was associated with an unfavorable outcome in patients with glioblastoma and GBM, and low NAGS expression was associated with an unfavorable outcome in patients with LUAD [1]. Inherited NAGS deficiency results in hyperammonemia, presumably due to the loss of CPSI activity [2].
In conclusion, NAGS is crucial for the urea cycle by activating CPSI through the production of NAG. Its abnormal expression is associated with certain tumors and its deficiency leads to hyperammonemia. Studies on NAGS contribute to understanding the mechanisms of tumorigenesis and urea cycle-related disorders.
References：
1. Owusu-Ansah, Melissa, Guptan, Nikita, Alindogan, Dylon, Morizono, Michio, Caldovic, Ljubica. 2023. NAGS, CPS1, and SLC25A13 (Citrin) at the Crossroads of Arginine and Pyrimidines Metabolism in Tumor Cells. In International journal of molecular sciences, 24, . doi:10.3390/ijms24076754. https://pubmed.ncbi.nlm.nih.gov/37047726/
2. Morizono, Hiroki, Caldovic, Ljubica, Shi, Dashuang, Tuchman, Mendel. . Mammalian N-acetylglutamate synthase. In Molecular genetics and metabolism, 81 Suppl 1, S4-11. doi:. https://pubmed.ncbi.nlm.nih.gov/15050968/
3. Shi, Dashuang, Allewell, Norma M, Tuchman, Mendel. 2015. The N-Acetylglutamate Synthase Family: Structures, Function and Mechanisms. In International journal of molecular sciences, 16, 13004-22. doi:10.3390/ijms160613004. https://pubmed.ncbi.nlm.nih.gov/26068232/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen