C57BL/6JCya-Tex2em1flox/Cya
Common Name:
Tex2-flox
Product ID:
S-CKO-06169
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tex2-flox
Strain ID
CKOCMP-21763-Tex2-B6J-VA
Gene Name
Product ID
S-CKO-06169
Gene Alias
4930568E07Rik; Def-5; Taz4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tex2em1flox/Cya mice (Catalog S-CKO-06169) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000042780
NCBI RefSeq
NM_198292
Target Region
Exon 3~4
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Tex2, also known as REPRESSOR OF CYTOKININ DEFICIENCY1 (ROCK1) in Arabidopsis, is a gene with diverse functions. It encodes a lipid transfer protein containing an SMP domain conserved in metazoan and yeast, and is involved in multiple biological pathways. In animals, it is crucial for lysosomal functions at TMEM55-dependent ER membrane contact sites, while in plants, it plays a role in pollen exine formation [1,2].
In functional studies, Tex2 knockout in mice leads to defects in lysosomal trafficking, digestive capacity, and lipid composition of late endosome/lysosome (LE/lys) membranes, highlighting its importance in lysosomal functions [1]. In Arabidopsis, loss of TEX2 results in abnormal primexine formation, lack of primary exine elements, and failure of sporopollenin to correctly assemble into exine structures, disrupting pollen exine formation [2].
In conclusion, Tex2 is essential for lysosomal functions in animals and pollen exine formation in plants. Gene knockout models in both mice and Arabidopsis have significantly contributed to understanding its role in these specific biological processes, providing valuable insights into related cellular mechanisms and potential disease or plant development-related implications.
References:
1. Du, Yuanjiao, Chang, Weiping, Gao, Lei, Deng, Lin, Ji, Wei-Ke. 2023. Tex2 is required for lysosomal functions at TMEM55-dependent ER membrane contact sites. In The Journal of cell biology, 222, . doi:10.1083/jcb.202205133. https://pubmed.ncbi.nlm.nih.gov/36705603/
2. Wang, Rui, Dobritsa, Anna A. . Loss of THIN EXINE2 disrupts multiple processes in the mechanism of pollen exine formation. In Plant physiology, 187, 133-157. doi:10.1093/plphys/kiab244. https://pubmed.ncbi.nlm.nih.gov/34618131/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen