C57BL/6JCya-Tfamem1flox/Cya
Common Name:
Tfam-flox
Product ID:
S-CKO-06197
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tfam-flox
Strain ID
CKOCMP-21780-Tfam-B6J-VA
Gene Name
Product ID
S-CKO-06197
Gene Alias
Hmgts; mtTFA; tsHMG
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tfamem1flox/Cya mice (Catalog S-CKO-06197) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000092430
NCBI RefSeq
NM_009360
Target Region
Exon 3~5
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
TFAM, also known as transcription factor A, mitochondrial, is a key transcription factor crucial for maintaining mitochondrial DNA (mtDNA) stabilization and initiating its replication [4]. It plays an essential role in cellular bioenergetics, as it is involved in mtDNA maintenance and expression, which is vital for cell survival [5]. TFAM is associated with pathways like autophagy, inflammation, and cGAS-STING pathway, and its function is studied using genetic models.
In multiple disease-related studies, TFAM shows significant impacts. In ischemic acute kidney injury, mitochondrial ROS (mtROS) promotes injury by suppressing TFAM-mediated mtDNA maintenance, while decreasing mtROS levels can reverse TFAM and mtDNA copy number decrease, suggesting TFAM could be a target for renal repair [1]. In alcoholic steatohepatitis, ATF4 activation promotes hepatic mitochondrial dysfunction by repressing NRF1-TFAM signalling, and TFAM overexpression can attenuate alcohol-induced mitochondrial dysfunction and liver damage [2]. In liver cancer, TFAM loss induces nuclear actin assembly upon mDia2 malonylation, promoting metastasis, and TFAM downregulation in metastatic tissues is associated with patient survival [3]. In dendritic cells, TFAM deficiency leads to mitochondrial dysfunction, mtDNA cytosolic leakage, cGAS-STING pathway activation, enhanced antigen presentation, and inhibition of tumor growth [4]. In esophageal squamous cell carcinoma (ESCC), TFAM downregulation promotes autophagy and ESCC survival through mtDNA stress-mediated STING pathway [6].
In conclusion, TFAM is essential for mitochondrial function and mtDNA maintenance. Gene-knockout models in mice have revealed its significant roles in various disease conditions such as kidney injury, liver diseases, cancer metastasis, and antitumor immunity. Understanding TFAM's functions through these models provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Zhao, Meng, Wang, Yizhuo, Li, Ling, Lu, Yanrong, Liu, Jingping. 2021. Mitochondrial ROS promote mitochondrial dysfunction and inflammation in ischemic acute kidney injury by disrupting TFAM-mediated mtDNA maintenance. In Theranostics, 11, 1845-1863. doi:10.7150/thno.50905. https://pubmed.ncbi.nlm.nih.gov/33408785/
2. Hao, Liuyi, Zhong, Wei, Dong, Haibo, Song, Zhenyuan, Zhou, Zhanxiang. 2020. ATF4 activation promotes hepatic mitochondrial dysfunction by repressing NRF1-TFAM signalling in alcoholic steatohepatitis. In Gut, 70, 1933-1945. doi:10.1136/gutjnl-2020-321548. https://pubmed.ncbi.nlm.nih.gov/33177163/
3. Huang, Qichao, Wu, Dan, Zhao, Jing, Liu, Xiaoli, Xing, Jinliang. 2022. TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis. In The EMBO journal, 41, e110324. doi:10.15252/embj.2021110324. https://pubmed.ncbi.nlm.nih.gov/35451091/
4. Lu, Tianqi, Zhang, Ziqi, Bi, Zhenfei, Luo, Min, Wei, Xiawei. . TFAM deficiency in dendritic cells leads to mitochondrial dysfunction and enhanced antitumor immunity through cGAS-STING pathway. In Journal for immunotherapy of cancer, 11, . doi:10.1136/jitc-2022-005430. https://pubmed.ncbi.nlm.nih.gov/36858460/
5. Kozhukhar, Natalya, Alexeyev, Mikhail F. 2023. 35 Years of TFAM Research: Old Protein, New Puzzles. In Biology, 12, . doi:10.3390/biology12060823. https://pubmed.ncbi.nlm.nih.gov/37372108/
6. Li, Yujia, Yang, Qi, Chen, Hui, Wang, Yanming, Bao, Dengke. 2022. TFAM downregulation promotes autophagy and ESCC survival through mtDNA stress-mediated STING pathway. In Oncogene, 41, 3735-3746. doi:10.1038/s41388-022-02365-z. https://pubmed.ncbi.nlm.nih.gov/35750756/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen