C57BL/6JCya-Kcnk13em1flox/Cya
Common Name:
Kcnk13-flox
Product ID:
S-CKO-06199
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Kcnk13-flox
Strain ID
CKOCMP-217826-Kcnk13-B6J-VA
Gene Name
Product ID
S-CKO-06199
Gene Alias
F730021E22Rik; Gm1570; Gm1685
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kcnk13em1flox/Cya mice (Catalog S-CKO-06199) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000049788
NCBI RefSeq
NM_001164427
Target Region
Exon 3
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Kcnk13, also known as THIK-1, encodes a two-pore potassium channel. This channel is involved in multiple biological functions. It plays a role in regulating microglial ramification, surveillance, and interleukin-1β release, and is also associated with the NLRP3-inflammasome pathway, which is important in inflammation-related processes [4,5].
In animal studies, knockdown of Kcnk13 in rats and mice has shown its significance in alcohol-related behaviors. In rats, knockdown of Kcnk13 using siRNA reduced ethanol-induced excitation of ventral tegmental area (VTA) neurons, and in mice, shRNA-mediated knockdown of Kcnk13 in the VTA increased alcohol drinking, indicating that Kcnk13 can control VTA neuronal activity and binge-like drinking [1,3]. Also, KCNK13 expression was increased by acute ethanol exposure in the VTA of mice [3].
In neurodegenerative diseases, the discovery of CVN293, a brain-permeable KCNK13 inhibitor, suggests that modulation of KCNK13 activity may be a potential treatment strategy, as KCNK13 is specifically expressed in human microglia with elevated expression in post-mortem human brain tissue from Alzheimer's disease patients [2].
In conclusion, Kcnk13 is a crucial gene involved in alcohol-related neuronal excitation and potential treatment of neurodegenerative diseases. Gene knockdown models in rats and mice have been instrumental in revealing its role in these processes, providing valuable insights for understanding the underlying mechanisms and developing potential therapeutic strategies.
References:
1. You, Chang, Vandegrift, Bertha J, Brodie, Mark S. 2021. KCNK13 potassium channels in the ventral tegmental area of rats are important for excitation of ventral tegmental area neurons by ethanol. In Alcoholism, clinical and experimental research, 45, 1348-1358. doi:10.1111/acer.14630. https://pubmed.ncbi.nlm.nih.gov/33960499/
2. Bürli, Roland W, Doyle, Kevin J, Dickson, Louise, Carlton, Mark, Dawson, Lee A. 2024. Discovery of CVN293, a Brain Permeable KCNK13 (THIK-1) Inhibitor Suitable for Clinical Assessment. In ACS medicinal chemistry letters, 15, 646-652. doi:10.1021/acsmedchemlett.4c00035. https://pubmed.ncbi.nlm.nih.gov/38746889/
3. You, Chang, Savarese, Antonia, Vandegrift, Bertha J, Lasek, Amy W, Brodie, Mark S. 2018. Ethanol acts on KCNK13 potassium channels in the ventral tegmental area to increase firing rate and modulate binge-like drinking. In Neuropharmacology, 144, 29-36. doi:10.1016/j.neuropharm.2018.10.008. https://pubmed.ncbi.nlm.nih.gov/30332606/
4. Madry, Christian, Kyrargyri, Vasiliki, Arancibia-Cárcamo, I Lorena, Bryan, Robert M, Attwell, David. 2017. Microglial Ramification, Surveillance, and Interleukin-1β Release Are Regulated by the Two-Pore Domain K+ Channel THIK-1. In Neuron, 97, 299-312.e6. doi:10.1016/j.neuron.2017.12.002. https://pubmed.ncbi.nlm.nih.gov/29290552/
5. Drinkall, Samuel, Lawrence, Catherine B, Ossola, Bernadino, Harte, Michael, Brough, David. 2022. The two pore potassium channel THIK-1 regulates NLRP3 inflammasome activation. In Glia, 70, 1301-1316. doi:10.1002/glia.24174. https://pubmed.ncbi.nlm.nih.gov/35353387/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen