C57BL/6JCya-Tgfaem1flox/Cya
Common Name:
Tgfa-flox
Product ID:
S-CKO-06224
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tgfa-flox
Strain ID
CKOCMP-21802-Tgfa-B6J-VA
Gene Name
Product ID
S-CKO-06224
Gene Alias
wa-1; wa1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tgfaem1flox/Cya mice (Catalog S-CKO-06224) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032066
NCBI RefSeq
NM_031199
Target Region
Exon 3
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Tgfa, also known as transforming growth factor alpha, is a polypeptide growth factor with a high affinity for the epidermal growth factor receptor. It is an essential cell regulator, acting during processes like cell proliferation, differentiation, migration, and apoptosis. Tgfa is involved in several signaling pathways, such as those frequently altered in hepatocellular carcinoma which involve growth factor receptors including Tgfa [2].
In cervical cancer, knockdown of Tgfa can inhibit the proliferation, migration, and invasion of cervical cancer cells. After Tgfa knockout, the expression of IL family and MMP family proteins in cervical cancer cell lines was significantly reduced, indicating its important role in the occurrence and development of cervical cancer [1].
In osteosarcoma, cisplatin inhibits the proliferation of Saos-2 cells by down-regulating Tgfa expression [3]. Also, in thyroid cancer, circRTN1 promotes cancer progression by upregulating Tgfa through sponging miR-431-5p [4].
In conclusion, Tgfa is a crucial cell regulator involved in multiple cellular processes. Model-based research, especially through gene knockdown and knockout experiments, has revealed its significant role in various cancers including cervical, osteosarcoma, and thyroid cancer. Understanding Tgfa's function provides potential targets for cancer treatment.
References：
1. Ma, Xiaoxuan, Zheng, Jingying, He, Kang, Zhao, Lijing, Wang, Lisheng. 2023. TGFA expression is associated with poor prognosis and promotes the development of cervical cancer. In Journal of cellular and molecular medicine, 28, e18086. doi:10.1111/jcmm.18086. https://pubmed.ncbi.nlm.nih.gov/38152044/
2. Garcia-Lezana, Teresa, Lopez-Canovas, Juan Luis, Villanueva, Augusto. 2020. Signaling pathways in hepatocellular carcinoma. In Advances in cancer research, 149, 63-101. doi:10.1016/bs.acr.2020.10.002. https://pubmed.ncbi.nlm.nih.gov/33579428/
3. Wang, Yuan, Wu, Yichao, Cai, Awei, Xu, Tangbing, Hu, Yong. 2021. Cisplatin inhibits the proliferation of Saos-2 osteosarcoma cells via the miR-376c/TGFA pathway. In Bosnian journal of basic medical sciences, 21, 163-173. doi:10.17305/bjbms.2020.4485. https://pubmed.ncbi.nlm.nih.gov/32020849/
4. Gao, Yu, Wang, Yichun, Xu, Lei, Zhu, Liyang, Wang, Fan. 2022. CircRTN1 acts as a miR-431-5p sponge to promote thyroid cancer progression by upregulating TGFA. In Hormones (Athens, Greece), 21, 611-623. doi:10.1007/s42000-022-00378-7. https://pubmed.ncbi.nlm.nih.gov/35804263/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen