C57BL/6JCya-Thy1em1flox/Cya
Common Name:
Thy1-flox
Product ID:
S-CKO-06276
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Thy1-flox
Strain ID
CKOCMP-21838-Thy1-B6J-VA
Gene Name
Product ID
S-CKO-06276
Gene Alias
CD90; T25; Thy-1; Thy-1.2; Thy1.1; Thy1.2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Thy1em1flox/Cya mice (Catalog S-CKO-06276) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000114840
NCBI RefSeq
NM_009382
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Thy1, also known as CD90, is a heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored cell surface protein. It plays diverse roles in various biological processes. It has been associated with pathways such as the regulation of adipogenesis, epithelial-mesenchymal transition (EMT), and adherens junction formation. Its dysregulation is related to diseases including cancer, obesity, and glomerulonephritis [1-4, 7-10]. Gene knockout (KO) mouse models can be valuable for studying its functions.
In the epidermis, Thy1 marks a distinct population of slow-cycling stem cells. Ablation of Thy1+ cells strongly impairs epidermal replenishment and wound repair, indicating their non-redundant function in epidermal homeostasis and repair [1]. In adipogenesis, DNA methylation regulates Thy1 expression, and Thy1-knockout mice are prone to diet-induced obesity [2]. In nasopharyngeal carcinoma, THY1 functions as a tumor suppressor, and its down-regulation is associated with tumor metastasis. Knockdown of THY1 disrupts adherens junctions and increases tumor invasion [3].
In conclusion, Thy1 has essential functions in epidermal maintenance, adipogenesis regulation, and tumor suppression. Studies using KO mouse models have revealed its significance in epidermal homeostasis, obesity-related processes, and cancer metastasis. These findings contribute to our understanding of the role of Thy1 in these specific disease areas.
References:
1. Koren, Elle, Feldman, Alona, Yusupova, Marianna, Shemesh, Tom, Fuchs, Yaron. 2022. Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis. In Nature communications, 13, 4628. doi:10.1038/s41467-022-31629-1. https://pubmed.ncbi.nlm.nih.gov/35941116/
2. Flores, E'Lissa M, Woeller, Collynn F, Falsetta, Megan L, Susiarjo, Martha, Phipps, Richard P. 2018. Thy1 (CD90) expression is regulated by DNA methylation during adipogenesis. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33, 3353-3363. doi:10.1096/fj.201801481R. https://pubmed.ncbi.nlm.nih.gov/30376360/
3. Chen, Luo, Chau, Wai Yin, Yuen, Hei Tung, Lung, Maria Li, Lung, Hong Lok. 2023. THY1 (CD90) Maintains the Adherens Junctions in Nasopharyngeal Carcinoma via Inhibition of SRC Activation. In Cancers, 15, . doi:10.3390/cancers15072189. https://pubmed.ncbi.nlm.nih.gov/37046850/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen