Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Tie1em1flox/Cya
Common Name:
Tie1-flox
Product ID:
S-CKO-06285
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tie1-flox
Strain ID
CKOCMP-21846-Tie1-B6J-VA
Gene Name
Tie1
Product ID
S-CKO-06285
Gene Alias
D430008P04Rik; TIE; tie-1
Background
C57BL/6JCya
NCBI ID
21846
Modification
Conditional knockout
Chromosome
4
Phenotype
MGI:99906
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tie1em1flox/Cya mice (Catalog S-CKO-06285) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000047421
NCBI RefSeq
NM_011587
Target Region
Exon 10~14
Size of Effective Region
~3.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Tie1, or Tyrosine kinase with immunoglobulin and EGF-like domains 1, is an endothelial cell-specific orphan receptor. It plays crucial roles in angiogenesis, lymphangiogenesis, and the development of the cardiovascular system. Tie1 modulates signaling pathways such as Angiopoietin/Tie2 signaling, and its associated pathways are involved in processes like cell migration, proliferation, and vessel morphogenesis [4,5,6,7]. Genetic models, including gene-knockout mouse models, have been instrumental in studying its functions.

In liver fibrosis, LECT2, a ligand of Tie1, interrupts Tie1/Tie2 heterodimerization, facilitating Tie2/Tie2 homodimerization, activating PPAR signaling, and inhibiting endothelial cell migration and tube formation. LECT2 overexpression in vivo inhibits portal angiogenesis, promotes sinusoid capillarization, and worsens fibrosis, while these changes are reversed in Lect2-KO mice [1]. In cervical cancer, TIE1 overexpression promotes cell proliferation, migration, invasion in vitro and tumor growth and metastasis in vivo, interacting with Basigin to stimulate the Basigin-matrix metalloproteinase axis [2]. In primary open-angle glaucoma, increased genetically proxied TIE1 signaling is associated with a reduction in intraocular pressure [3]. In zebrafish, tie1 mutant embryos display reduced endothelial and endocardial cell numbers, reduced heart size, and cardiac defects, with down-regulation of tll1, and tll1 overexpression can partially rescue these phenotypes [4]. In mice, TIE1 deficiency disrupts venous integrity, leading to increased sprouting angiogenesis and vascular bleeding, and TIE1 and TIE2 act synergistically with COUP-TFII to restrict sprouting angiogenesis during venous system development [5]. Loss-of-function mutations of TIE1 in humans and homozygous mouse models cause lymphatic dysfunction and edema, emphasizing the role of the ANGPT2/TIE1 pathway in lymphatic function [6]. In zebrafish, Angpt1 binds to Tie1 as a ligand, and Angpt1/Tie1 signaling is essential for lymphatic development, functioning in endothelial cell migration, proliferation, and lymphatic specification [7].

In conclusion, Tie1 is essential for multiple biological processes including angiogenesis, lymphangiogenesis, and cardiovascular development. Model-based research, especially KO mouse models, has revealed its role in various disease conditions such as liver fibrosis, cervical cancer, primary open-angle glaucoma, and lymphatic-related disorders, providing potential therapeutic targets for these diseases.

References:
1. Xu, Meng, Xu, Hong-Hai, Lin, Yuan, Ding, Yan-Qing, Zhou, Wei-Jie. 2019. LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis. In Cell, 178, 1478-1492.e20. doi:10.1016/j.cell.2019.07.021. https://pubmed.ncbi.nlm.nih.gov/31474362/
2. Liu, Pan, Xie, Lisha, Wu, Qiulei, Yang, Ping, Cai, Liqiong. 2024. TIE1 promotes cervical cancer progression via Basigin-matrix metalloproteinase axis. In International journal of biological sciences, 20, 2297-2309. doi:10.7150/ijbs.93667. https://pubmed.ncbi.nlm.nih.gov/38617545/
3. Rajasundaram, Skanda, Zebardast, Nazlee, Mehta, Puja, Segrè, Ayellet V, Wiggs, Janey. 2023. TIE1 and TEK signalling, intraocular pressure, and primary open-angle glaucoma: a Mendelian randomization study. In Journal of translational medicine, 21, 847. doi:10.1186/s12967-023-04737-9. https://pubmed.ncbi.nlm.nih.gov/37996923/
4. Carlantoni, Claudia, Allanki, Srinivas, Kontarakis, Zacharias, Günther, Stefan, Stainier, Didier Y R. 2020. Tie1 regulates zebrafish cardiac morphogenesis through Tolloid-like 1 expression. In Developmental biology, 469, 54-67. doi:10.1016/j.ydbio.2020.09.008. https://pubmed.ncbi.nlm.nih.gov/32971120/
5. Cao, Xudong, Li, Taotao, Xu, Beibei, Kubota, Yoshiaki, He, Yulong. 2023. Endothelial TIE1 Restricts Angiogenic Sprouting to Coordinate Vein Assembly in Synergy With Its Homologue TIE2. In Arteriosclerosis, thrombosis, and vascular biology, 43, e323-e338. doi:10.1161/ATVBAHA.122.318860. https://pubmed.ncbi.nlm.nih.gov/37317851/
6. Brouillard, Pascal, Murtomäki, Aino, Leppänen, Veli-Matti, Alitalo, Kari, Vikkula, Miikka. 2024. Loss-of-function mutations of the TIE1 receptor tyrosine kinase cause late-onset primary lymphedema. In The Journal of clinical investigation, 134, . doi:10.1172/JCI173586. https://pubmed.ncbi.nlm.nih.gov/38820174/
7. Morooka, Nanami, Gui, Ning, Ando, Koji, Mochizuki, Naoki, Nakajima, Hiroyuki. 2024. Angpt1 binding to Tie1 regulates the signaling required for lymphatic vessel development in zebrafish. In Development (Cambridge, England), 151, . doi:10.1242/dev.202269. https://pubmed.ncbi.nlm.nih.gov/38742432/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest