C57BL/6JCya-Tie1em1flox/Cya
Common Name:
Tie1-flox
Product ID:
S-CKO-06285
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tie1-flox
Strain ID
CKOCMP-21846-Tie1-B6J-VA
Gene Name
Product ID
S-CKO-06285
Gene Alias
D430008P04Rik; TIE; tie-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tie1em1flox/Cya mice (Catalog S-CKO-06285) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000047421
NCBI RefSeq
NM_011587
Target Region
Exon 10~14
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Tie1, or Tyrosine kinase with immunoglobulin and EGF-like domains 1, is an endothelial cell-specific orphan receptor. It plays crucial roles in angiogenesis, lymphangiogenesis, and the development of the cardiovascular system. Tie1 modulates signaling pathways such as Angiopoietin/Tie2 signaling, and its associated pathways are involved in processes like cell migration, proliferation, and vessel morphogenesis [4,5,6,7]. Genetic models, including gene-knockout mouse models, have been instrumental in studying its functions.
In liver fibrosis, LECT2, a ligand of Tie1, interrupts Tie1/Tie2 heterodimerization, facilitating Tie2/Tie2 homodimerization, activating PPAR signaling, and inhibiting endothelial cell migration and tube formation. LECT2 overexpression in vivo inhibits portal angiogenesis, promotes sinusoid capillarization, and worsens fibrosis, while these changes are reversed in Lect2-KO mice [1]. In cervical cancer, TIE1 overexpression promotes cell proliferation, migration, invasion in vitro and tumor growth and metastasis in vivo, interacting with Basigin to stimulate the Basigin-matrix metalloproteinase axis [2]. In primary open-angle glaucoma, increased genetically proxied TIE1 signaling is associated with a reduction in intraocular pressure [3]. In zebrafish, tie1 mutant embryos display reduced endothelial and endocardial cell numbers, reduced heart size, and cardiac defects, with down-regulation of tll1, and tll1 overexpression can partially rescue these phenotypes [4]. In mice, TIE1 deficiency disrupts venous integrity, leading to increased sprouting angiogenesis and vascular bleeding, and TIE1 and TIE2 act synergistically with COUP-TFII to restrict sprouting angiogenesis during venous system development [5]. Loss-of-function mutations of TIE1 in humans and homozygous mouse models cause lymphatic dysfunction and edema, emphasizing the role of the ANGPT2/TIE1 pathway in lymphatic function [6]. In zebrafish, Angpt1 binds to Tie1 as a ligand, and Angpt1/Tie1 signaling is essential for lymphatic development, functioning in endothelial cell migration, proliferation, and lymphatic specification [7].
In conclusion, Tie1 is essential for multiple biological processes including angiogenesis, lymphangiogenesis, and cardiovascular development. Model-based research, especially KO mouse models, has revealed its role in various disease conditions such as liver fibrosis, cervical cancer, primary open-angle glaucoma, and lymphatic-related disorders, providing potential therapeutic targets for these diseases.
References:
1. Xu, Meng, Xu, Hong-Hai, Lin, Yuan, Ding, Yan-Qing, Zhou, Wei-Jie. 2019. LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis. In Cell, 178, 1478-1492.e20. doi:10.1016/j.cell.2019.07.021. https://pubmed.ncbi.nlm.nih.gov/31474362/
2. Liu, Pan, Xie, Lisha, Wu, Qiulei, Yang, Ping, Cai, Liqiong. 2024. TIE1 promotes cervical cancer progression via Basigin-matrix metalloproteinase axis. In International journal of biological sciences, 20, 2297-2309. doi:10.7150/ijbs.93667. https://pubmed.ncbi.nlm.nih.gov/38617545/
3. Rajasundaram, Skanda, Zebardast, Nazlee, Mehta, Puja, Segrè, Ayellet V, Wiggs, Janey. 2023. TIE1 and TEK signalling, intraocular pressure, and primary open-angle glaucoma: a Mendelian randomization study. In Journal of translational medicine, 21, 847. doi:10.1186/s12967-023-04737-9. https://pubmed.ncbi.nlm.nih.gov/37996923/
4. Carlantoni, Claudia, Allanki, Srinivas, Kontarakis, Zacharias, Günther, Stefan, Stainier, Didier Y R. 2020. Tie1 regulates zebrafish cardiac morphogenesis through Tolloid-like 1 expression. In Developmental biology, 469, 54-67. doi:10.1016/j.ydbio.2020.09.008. https://pubmed.ncbi.nlm.nih.gov/32971120/
5. Cao, Xudong, Li, Taotao, Xu, Beibei, Kubota, Yoshiaki, He, Yulong. 2023. Endothelial TIE1 Restricts Angiogenic Sprouting to Coordinate Vein Assembly in Synergy With Its Homologue TIE2. In Arteriosclerosis, thrombosis, and vascular biology, 43, e323-e338. doi:10.1161/ATVBAHA.122.318860. https://pubmed.ncbi.nlm.nih.gov/37317851/
6. Brouillard, Pascal, Murtomäki, Aino, Leppänen, Veli-Matti, Alitalo, Kari, Vikkula, Miikka. 2024. Loss-of-function mutations of the TIE1 receptor tyrosine kinase cause late-onset primary lymphedema. In The Journal of clinical investigation, 134, . doi:10.1172/JCI173586. https://pubmed.ncbi.nlm.nih.gov/38820174/
7. Morooka, Nanami, Gui, Ning, Ando, Koji, Mochizuki, Naoki, Nakajima, Hiroyuki. 2024. Angpt1 binding to Tie1 regulates the signaling required for lymphatic vessel development in zebrafish. In Development (Cambridge, England), 151, . doi:10.1242/dev.202269. https://pubmed.ncbi.nlm.nih.gov/38742432/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen