C57BL/6JCya-Chdhem1flox/Cya
Common Name
Chdh-flox
Product ID
S-CKO-06329
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-218865-Chdh-B6J-VA
When using this mouse strain in a publication, please cite “Chdh-flox Mouse (Catalog S-CKO-06329) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Chdh-flox
Strain ID
CKOCMP-218865-Chdh-B6J-VA
Gene Name
Product ID
S-CKO-06329
Gene Alias
D630034H06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000067620
NCBI RefSeq
NM_001136240
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Overview of Gene Research
Chdh, or choline dehydrogenase, is a transmembrane protein located in mitochondria. It is a key enzyme that catalyzes the oxidation of choline to betaine, playing a crucial role in this metabolic pathway. Betaine is an important methyl donor and organic osmolyte, and thus Chdh is vital for cellular physiology and metabolism. Additionally, Chdh is involved in mitochondrial autophagy (mitophagy) after mitochondrial damage [1,3].
In a Chdh knockout (KO) mouse model, deletion of Chdh did not affect fetal viability, growth, or survival of the mice. However, only one of eleven Chdh -/- males was able to reproduce, as loss of CHDH activity led to decreased testicular betaine and increased choline and PCho concentrations. The impaired fertility was due to diminished sperm motility in Chdh -/- males, along with abnormal mitochondrial morphology in their sperm. ATP content, total mitochondrial dehydrogenase activity, and inner mitochondrial membrane polarization were all significantly reduced in sperm from Chdh -/- animals. Mitochondrial changes were also detected in liver, kidney, heart, and testis tissues [2].
In conclusion, Chdh is essential for the conversion of choline to betaine and for maintaining normal mitochondrial function and sperm motility. The Chdh KO mouse model has revealed its significance in male fertility and mitochondrial-related functions in various tissues, providing valuable insights into potential disease mechanisms related to Chdh dysfunction, such as infertility [2].
References：
1. Li, Yifei, Shen, Xinzhuang, Yang, Xiaowen, Shen, Wenzhi, Liu, Huan. 2023. CHDH, a key mitochondrial enzyme, plays a diagnostic role in metabolic disorders diseases and tumor progression. In Frontiers in genetics, 14, 1240650. doi:10.3389/fgene.2023.1240650. https://pubmed.ncbi.nlm.nih.gov/37600654/
2. Johnson, Amy R, Craciunescu, Corneliu N, Guo, Zhong, Blusztajn, Jan K, Zeisel, Steven H. 2010. Deletion of murine choline dehydrogenase results in diminished sperm motility. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24, 2752-61. doi:10.1096/fj.09-153718. https://pubmed.ncbi.nlm.nih.gov/20371614/
3. Park, Sungwoo, Choi, Seon-Guk, Yoo, Seung-Min, Son, Jin H, Jung, Yong-Keun. 2014. Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy. In Autophagy, 10, 1906-20. doi:10.4161/auto.32177. https://pubmed.ncbi.nlm.nih.gov/25483962/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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