C57BL/6JCya-Tlr1em1flox/Cya
Common Name:
Tlr1-flox
Product ID:
S-CKO-06337
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tlr1-flox
Strain ID
CKOCMP-21897-Tlr1-B6J-VA
Gene Name
Product ID
S-CKO-06337
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tlr1em1flox/Cya mice (Catalog S-CKO-06337) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000197315
NCBI RefSeq
NM_001276445
Target Region
Exon 4
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Tlr1, Toll-like receptor 1, is a key component in the innate immune system. It often forms heterodimers with Tlr2 and recognizes various microbial ligands, especially bacterial lipoproteins. This recognition activates signaling cascades such as the MAPK and NF-κB pathways, playing a crucial role in initiating immune responses against pathogens [2,3].
Lactobacillus johnsonii, a potential anti-inflammatory bacterium, can alleviate colitis through the Tlr1/2-STAT3 mediated activation of CD206+ macrophages-IL-10. Tlr1/2 is essential for the activation of STAT3 and the recognition of L. johnsonii by macrophages, suggesting its role in modulating gut microbiota-related immune responses in ulcerative colitis [1]. In hybrid yellow catfish, the expression of Tlr1 is up-regulated after Aeromonas hydrophila stimulation, indicating its participation in the inflammatory response to exogenous pathogen infection [4].
In conclusion, Tlr1 is vital for the innate immune response, recognizing microbial ligands and activating relevant signaling pathways. Its role in diseases like ulcerative colitis and in response to pathogen infections in fish has been revealed through in vivo studies, highlighting its significance in understanding immune-related disease mechanisms and potentially developing new therapeutic strategies.
References:
1. Jia, Ding-Jia-Cheng, Wang, Qi-Wen, Hu, Ying-Ying, Chen, Shu-Jie, Wang, Liang-Jing. . Lactobacillus johnsonii alleviates colitis by TLR1/2-STAT3 mediated CD206+ macrophagesIL-10 activation. In Gut microbes, 14, 2145843. doi:10.1080/19490976.2022.2145843. https://pubmed.ncbi.nlm.nih.gov/36398889/
2. Raieli, Salvatore, Trichot, Coline, Korniotis, Sarantis, Pattarini, Lucia, Soumelis, Vassili. 2019. TLR1/2 orchestrate human plasmacytoid predendritic cell response to gram+ bacteria. In PLoS biology, 17, e3000209. doi:10.1371/journal.pbio.3000209. https://pubmed.ncbi.nlm.nih.gov/31017904/
3. Noreen, Mamoona, Arshad, Muhammad. . Association of TLR1, TLR2, TLR4, TLR6, and TIRAP polymorphisms with disease susceptibility. In Immunologic research, 62, 234-52. doi:10.1007/s12026-015-8640-6. https://pubmed.ncbi.nlm.nih.gov/25784622/
4. Guo, Shengtao, Gao, Wenxue, Zeng, Mengsha, Shi, Qiong, Song, Zhaobin. 2023. Characterization of TLR1 and expression profiling of TLR signaling pathway related genes in response to Aeromonas hydrophila challenge in hybrid yellow catfish (Pelteobagrus fulvidraco ♀ × P. vachelli ♂). In Frontiers in immunology, 14, 1163781. doi:10.3389/fimmu.2023.1163781. https://pubmed.ncbi.nlm.nih.gov/37056759/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen