C57BL/6JCya-Ccar2em1flox/Cya
Common Name
Ccar2-flox
Product ID
S-CKO-06367
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-219158-Ccar2-B6J-VA
Status
When using this mouse strain in a publication, please cite “Ccar2-flox Mouse (Catalog S-CKO-06367) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ccar2-flox
Strain ID
CKOCMP-219158-Ccar2-B6J-VA
Gene Name
Product ID
S-CKO-06367
Gene Alias
Dbc1, mKIAA1967, 2610301G19Rik
Background
C57BL/6JCya
Gene Full Name
cell cycle activator and apoptosis regulator 2
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 14 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000035612
NCBI Transcript ID
NM_146055
Target Region
Exon 4~7
Size of Effective Region
~1.5 kb
Overview of Gene Research
CCAR2, also known as DBC1 (deleted in breast cancer 1), is a multifaceted protein involved in diverse physiological and pathophysiological processes [2,4]. It acts as a coregulator of various transcription factors and a critical regulator of numerous epigenetic modifiers, playing roles in apoptosis, DNA repair, metabolism, and tumorigenesis [2]. It is part of the 53BP1-RIF1 pathway which restricts DNA double-strand break (DSB) resection and promotes non-homologous end joining (NHEJ) repair [1].
Gene knockout studies have provided significant insights into CCAR2 functions. In a BRCA1-deficient cell line, knockout of CCAR2 led to elevated DSB end-resection, RAD51 loading, and PARP inhibitor (PARPi) resistance, indicating its role in suppressing homologous recombination [1]. CCAR2-deficient cells also showed defects in cell division, with perturbed mitotic phase, premature loss of centromere cohesion, and activation of the abscission checkpoint, suggesting it governs mitotic events to maintain chromosomal stability [3]. In osteosarcoma cells, silencing CCAR2 prevented the malignant phenotype both in vitro and in nude mice [5].
In conclusion, CCAR2 plays essential roles in DNA repair, cell cycle regulation, and cancer-related processes. Gene knockout models have been crucial in revealing its functions, especially in understanding DNA repair mechanisms and cancer-associated phenotypes such as in osteosarcoma, providing potential targets for therapeutic interventions [1,3,5].
References：
1. Iyer, Divya Ramalingam, Harada, Naoya, Clairmont, Connor, Chowdhury, Dipanjan, D'Andrea, Alan D. 2022. CCAR2 functions downstream of the Shieldin complex to promote double-strand break end-joining. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2214935119. doi:10.1073/pnas.2214935119. https://pubmed.ncbi.nlm.nih.gov/36442094/
2. Kim, Hwa Jin, Moon, Sue Jin, Kim, Jeong Hoon. 2023. Mechanistic insights into the dual role of CCAR2/DBC1 in cancer. In Experimental & molecular medicine, 55, 1691-1701. doi:10.1038/s12276-023-01058-1. https://pubmed.ncbi.nlm.nih.gov/37524873/
3. Ryu, Jaewook, Kim, Ja-Eun. 2022. CCAR2 controls mitotic progression through spatiotemporal regulation of Aurora B. In Cell death & disease, 13, 534. doi:10.1038/s41419-022-04990-8. https://pubmed.ncbi.nlm.nih.gov/35672287/
4. Johnson, Gavin S, Rajendran, Praveen, Dashwood, Roderick H. 2020. CCAR1 and CCAR2 as gene chameleons with antagonistic duality: Preclinical, human translational, and mechanistic basis. In Cancer science, 111, 3416-3425. doi:10.1111/cas.14579. https://pubmed.ncbi.nlm.nih.gov/33403784/
5. Chen, Liang, Zhou, Shi-Jin, Xu, Yan, Zou, Yin-Shuang, Pei, Hong. 2021. CCAR2 promotes a malignant phenotype of osteosarcoma through Wnt/β-catenin-dependent transcriptional activation of SPARC. In Biochemical and biophysical research communications, 580, 67-73. doi:10.1016/j.bbrc.2021.09.070. https://pubmed.ncbi.nlm.nih.gov/34624572/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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