C57BL/6JCya-Tnfsf11em1flox/Cya
Common Name:
Tnfsf11-flox
Product ID:
S-CKO-06394
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tnfsf11-flox
Strain ID
CKOCMP-21943-Tnfsf11-B6J-VA
Gene Name
Product ID
S-CKO-06394
Gene Alias
Ly109l; ODF; OPGL; RANKL; Trance
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tnfsf11em1flox/Cya mice (Catalog S-CKO-06394) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022592
NCBI RefSeq
NM_011613
Target Region
Exon 3~4
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Tnfsf11, also known as RANKL (Receptor Activator of NF-κB Ligand), is a key cytokine in the RANKL/RANK/OPG pathway. It is crucial for osteoclast differentiation and activation, playing a significant role in bone metabolism [2]. Dysregulation of this pathway can lead to various bone-related diseases. Additionally, it is involved in immune-related processes during pregnancy and in the development of certain cancers [3,4].
In lung adenocarcinoma, overexpression of Tnfsf11 reduces GPX4 levels and increases sensitivity to ferroptosis inducers. Gene knockdown or overexpression experiments demonstrated that Tnfsf11 negatively regulates GPX4, providing potential therapeutic targets for this malignancy [1].
In preeclampsia, polymorphisms in the Tnfsf11 gene (rs2200287 and rs2148072) were associated with the disease, suggesting its role in the immune-related aspects of pregnancy [3].
In membranous nephropathy, the miR-149-5p/TNFSF11 pathway was found to be involved in angiotensin II-induced podocyte apoptosis, where TNFSF11 upregulation increased apoptosis under angiotensin II stimulation [5].
In conclusion, Tnfsf11 is essential for osteoclast-mediated bone resorption and has implications in multiple disease areas such as lung adenocarcinoma, preeclampsia, and membranous nephropathy. Functional studies, including gene knockdown experiments, have revealed its regulatory role in key cellular processes within these diseases, highlighting its potential as a therapeutic target.
References:
1. Li, Zizhen, Lu, Wenhua, Yin, Feng, Li, Heping, Huang, Amin. 2024. Overexpression of TNFSF11 reduces GPX4 levels and increases sensitivity to ferroptosis inducers in lung adenocarcinoma. In Journal of translational medicine, 22, 340. doi:10.1186/s12967-024-05112-y. https://pubmed.ncbi.nlm.nih.gov/38594779/
2. Boyle, William J, Simonet, W Scott, Lacey, David L. . Osteoclast differentiation and activation. In Nature, 423, 337-42. doi:. https://pubmed.ncbi.nlm.nih.gov/12748652/
3. Sivaraj, Nagarjuna, Kusuma, Bunga Papa, Kutikuppala, L V Simhachalam, Balaga, Vijaya Sirisha, Gundakaram, Samhitha. 2022. Association of TNFSF11 rs2200287 and TNFSF11 rs2148072 gene polymorphisms in preeclampsia. In American journal of reproductive immunology (New York, N.Y. : 1989), 88, e13604. doi:10.1111/aji.13604. https://pubmed.ncbi.nlm.nih.gov/35869907/
4. Li, Pengfei, Li, Ying, Bai, Shuping, Zhang, Yu, Zhao, Ling. 2023. miR-4732-3p prevents lung cancer progression via inhibition of the TBX15/TNFSF11 axis. In Epigenomics, 15, 195-207. doi:10.2217/epi-2023-0009. https://pubmed.ncbi.nlm.nih.gov/37125501/
5. Qiu, Donghao, Zhao, Ning, Chen, Qi, Wang, Ming. 2022. Knockdown of circ_CDYL Contributes to Inhibit Angiotensin II-Induced Podocytes Apoptosis in Membranous Nephropathy via the miR-149-5p/TNFSF11 Pathway. In Journal of cardiovascular pharmacology, 79, 887-895. doi:10.1097/FJC.0000000000001262. https://pubmed.ncbi.nlm.nih.gov/35353073/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen