C57BL/6JCya-Tnfsf9em1flox/Cya
Common Name:
Tnfsf9-flox
Product ID:
S-CKO-06398
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tnfsf9-flox
Strain ID
CKOCMP-21950-Tnfsf9-B6J-VA
Gene Name
Product ID
S-CKO-06398
Gene Alias
4-1BB-L; 4-1BBL; Cd137l; Ly63l
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tnfsf9em1flox/Cya mice (Catalog S-CKO-06398) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000039490
NCBI RefSeq
NM_009404
Target Region
Exon 3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Tnfsf9, also known as CD137L/4-1BBL, is a member of the tumor necrosis factor superfamily. It functions as a T-cell co-stimulator, playing a role in cancer immunotherapy and being involved in regulating immune cell functions. It has implications in various biological processes related to the immune response and cancer [1,7].
In pancreatic cancer, Tnfsf9 expression is elevated and is associated with poor prognosis. It can promote pancreatic cancer metastasis by inducing M2 macrophage polarization through Src/FAK/p-Akt/IL-1β signaling [3], as well as through Wnt/Snail signaling [4].
In glioma, hypoxia can upregulate Tnfsf9 expression in a histone-lactylation-dependent manner, leading to M2 macrophage polarization and promoting glioma progression [2].
In hepatocellular carcinoma, Tnfsf9 expression is downregulated, and overexpression or treatment with its recombinant protein can inhibit HCC cell proliferation, migration, and invasion both in vitro and in vivo, suggesting it may be a tumor suppressor [7].
In patients with metastatic renal cell carcinoma treated with nivolumab and ipilimumab, Tnfsf9 expression may predict treatment response, with high-expression tumors showing an activated adaptive immune response [5].
Inherited Tnfsf9 deficiency in a patient led to broad Epstein-Barr virus infection with EBV+ smooth muscle tumors, highlighting the role of the CD137-CD137L (Tnfsf9) pathway in anti-EBV immunity [6].
In conclusion, Tnfsf9 has diverse functions in the immune response and cancer. Its role in promoting or inhibiting tumor progression varies depending on the cancer type. Studies on Tnfsf9, including those using relevant models, have provided insights into its functions in diseases such as cancer and viral infections, which may help develop new therapeutic strategies.
References：
1. Wu, Jiao, Wang, Yunpeng. 2022. Role of TNFSF9 bidirectional signal transduction in antitumor immunotherapy. In European journal of pharmacology, 928, 175097. doi:10.1016/j.ejphar.2022.175097. https://pubmed.ncbi.nlm.nih.gov/35714694/
2. Li, Min, Sun, Pingfeng, Tu, Binfeng, Li, Donghai, He, Wei. 2024. Hypoxia conduces the glioma progression by inducing M2 macrophage polarization via elevating TNFSF9 level in a histone-lactylation-dependent manner. In American journal of physiology. Cell physiology, 327, C487-C504. doi:10.1152/ajpcell.00124.2024. https://pubmed.ncbi.nlm.nih.gov/39010835/
3. Wu, Jiao, Wang, Yunpeng, Yang, Yichun, Jiang, Zhongxiang, Jiang, Zheng. 2021. TNFSF9 promotes metastasis of pancreatic cancer by regulating M2 polarization of macrophages through Src/FAK/p-Akt/IL-1β signaling. In International immunopharmacology, 102, 108429. doi:10.1016/j.intimp.2021.108429. https://pubmed.ncbi.nlm.nih.gov/34906856/
4. Wu, Jiao, Wang, Yunpeng, Yang, Yichun, Jiang, Zhongxiang, Jiang, Zheng. 2021. TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages. In Aging, 13, 21571-21586. doi:10.18632/aging.203497. https://pubmed.ncbi.nlm.nih.gov/34517345/
5. Isoda, Bunpei, Kandori, Shuya, Sazuka, Tomokazu, Ichikawa, Tomohiko, Nishiyama, Hiroyuki. 2024. TNFSF9 Is Associated with Favorable Tumor Immune Microenvironment in Patients with Renal Cell Carcinoma Who Are Treated with the Combination Therapy of Nivolumab and Ipilimumab. In International journal of molecular sciences, 25, . doi:10.3390/ijms25137444. https://pubmed.ncbi.nlm.nih.gov/39000552/
6. Fournier, Benjamin, Hoshino, Akihiro, Bruneau, Julie, Neven, Bénédicte, Latour, Sylvain. 2022. Inherited TNFSF9 deficiency causes broad Epstein-Barr virus infection with EBV+ smooth muscle tumors. In The Journal of experimental medicine, 219, . doi:10.1084/jem.20211682. https://pubmed.ncbi.nlm.nih.gov/35657354/
7. Shen, Yu Ling, Gan, Yu, Gao, Hai Feng, Wang, Jia Dong, Tu, Hong. . TNFSF9 exerts an inhibitory effect on hepatocellular carcinoma. In Journal of digestive diseases, 18, 395-403. doi:10.1111/1751-2980.12489. https://pubmed.ncbi.nlm.nih.gov/28547807/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen