C57BL/6JCya-Tns1em1flox/Cya
Common Name:
Tns1-flox
Product ID:
S-CKO-06409
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tns1-flox
Strain ID
CKOCMP-21961-Tns1-B6J-VA
Gene Name
Product ID
S-CKO-06409
Gene Alias
1110018I21Rik; 1200014E20Rik; E030018G17Rik; E030037J05Rik; Tns
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tns1em1flox/Cya mice (Catalog S-CKO-06409) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000169786
NCBI RefSeq
NM_027884
Target Region
Exon 16
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Tns1, short for tensin1, is a member of the tensin family, which are cellular-adhesion constituents. Tns1 engages in multiple biological processes such as cell adhesion, polarization, migration, invasion, proliferation, apoptosis, and mechano-transduction by interacting with various partner proteins. Abnormal Tns1 expression in vivo is associated with the development of various diseases, especially tumors. Genetic models, like gene knockout models, are valuable for studying Tns1 [1].
Tns1-knockout MDCK cells and mouse models have been used to study cystic kidney disease. Tns1-knockout MDCK cells form cysts with multiple lumens and have upregulated Mek/Erk activities. Re-expression of wild-type Tns1, but not a mutant lacking a cell-cell junction localization fragment, can rescue this phenotype. Mek inhibitor treatments can also restore the multiple lumens to single-lumen cysts. In Tns1-knockout mouse kidneys, treatment with the Mek inhibitor trametinib significantly reduces interstitial infiltrates, fibrosis, and dilated tubules, providing potential therapeutic strategies for cystic kidney diseases [2].
In conclusion, Tns1 is crucial for various biological processes, especially those related to cell behavior. Tns1-knockout models have revealed its role in cystic kidney disease, highlighting its potential as a therapeutic target in this disease area. Additionally, its abnormal expression is linked to tumor development, indicating its importance in understanding cancer biology as well [1,2].
References:
1. Wang, Zhihui, Ye, Jingxue, Dong, Fengrui, Wang, Min, Sun, Guibo. 2022. TNS1: Emerging Insights into Its Domain Function, Biological Roles, and Tumors. In Biology, 11, . doi:10.3390/biology11111571. https://pubmed.ncbi.nlm.nih.gov/36358270/
2. Wu, Zong-Ye, Chiu, Chun-Lung, Lo, Ethan, Yamada, Soichiro, Lo, Su Hao. 2019. Hyperactivity of Mek in TNS1 knockouts leads to potential treatments for cystic kidney diseases. In Cell death & disease, 10, 871. doi:10.1038/s41419-019-2119-7. https://pubmed.ncbi.nlm.nih.gov/31740667/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen