C57BL/6JCya-Tph1em1flox/Cya
Common Name:
Tph1-flox
Product ID:
S-CKO-06421
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tph1-flox
Strain ID
CKOCMP-21990-Tph1-B6J-VA
Gene Name
Product ID
S-CKO-06421
Gene Alias
Tph
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tph1em1flox/Cya mice (Catalog S-CKO-06421) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000049298
NCBI RefSeq
NM_009414
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Tph1, the rate-limiting enzyme in serotonin synthesis in certain cell types, is mainly expressed by specialized gut endocrine cells (enterochromaffin cells) and other non-neuronal cell types like adipocytes [3]. Serotonin, synthesized from tryptophan by Tph1, has a wide range of physiological functions, including roles in mood, sleep, anxiety, and gastrointestinal motility regulation, and also in glucose homeostasis and adiposity [3]. Tph1-related research can provide insights into these physiological processes and associated diseases.
In the context of depression, berberine improves depressive symptoms in CUMS-stimulated mice by activating Tph1, promoting tryptophan biotransformation into serotonin and inhibiting the kynurenine metabolism pathway [1]. Regarding suicide behavior, a meta-analysis suggests that the A218C polymorphism of Tph1 gene could be a possible risk factor [2]. In bladder cancer, increasing Tph1 activity inhibits the survival and proliferation of cells via the HIF-1α pathway, indicating Tph1 may be a potential therapeutic target [4]. In pancreatic ductal adenocarcinoma, the overexpression of Tph1 is related to gemcitabine resistance, and knocking down Tph1 can reduce cancer stem cells and reverse resistance [5].
In conclusion, Tph1 is crucial for serotonin synthesis and plays important roles in multiple physiological and disease-related processes. Research on Tph1, especially through genetic models, helps to understand its functions in depression, suicide behavior, bladder cancer, and pancreatic ductal adenocarcinoma, providing potential directions for disease treatment and prevention.
References:
1. Ge, Ping-Yuan, Qu, Shu-Yue, Ni, Sai-Jia, Zhang, Qi-Chun, Zhu, Hua-Xu. 2022. Berberine ameliorates depression-like behavior in CUMS mice by activating TPH1 and inhibiting IDO1-associated with tryptophan metabolism. In Phytotherapy research : PTR, 37, 342-357. doi:10.1002/ptr.7616. https://pubmed.ncbi.nlm.nih.gov/36089660/
2. Genis-Mendoza, Alma Delia, Hernández-Díaz, Yazmín, González-Castro, Thelma Beatriz, Ramos-Méndez, Miguel Ángel, Nicolini, Humberto. 2022. Association between TPH1 polymorphisms and the risk of suicide behavior: An updated meta-analysis of 18,398 individuals. In Frontiers in psychiatry, 13, 932135. doi:10.3389/fpsyt.2022.932135. https://pubmed.ncbi.nlm.nih.gov/35928776/
3. Jones, Lauren A, Sun, Emily W, Martin, Alyce M, Keating, Damien J. 2020. The ever-changing roles of serotonin. In The international journal of biochemistry & cell biology, 125, 105776. doi:10.1016/j.biocel.2020.105776. https://pubmed.ncbi.nlm.nih.gov/32479926/
4. Ren, Jianwei, Mo, Zhiting, Deng, Xia, Jin, Jie, Zhang, Huihui. 2024. TPH1 inhibits bladder tumorigenesis by targeting HIF-1α pathway in bladder cancer. In Asian biomedicine : research, reviews and news, 18, 171-179. doi:10.2478/abm-2024-0023. https://pubmed.ncbi.nlm.nih.gov/39309474/
5. Chaudhary, Prakash, Guragain, Diwakar, Chang, Jae-Hoon, Kim, Jung-Ae. 2021. TPH1 and 5-HT7 Receptor Overexpression Leading to Gemcitabine-Resistance Requires Non-Canonical Permissive Action of EZH2 in Pancreatic Ductal Adenocarcinoma. In Cancers, 13, . doi:10.3390/cancers13215305. https://pubmed.ncbi.nlm.nih.gov/34771469/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen