C57BL/6JCya-Hsp90b1em1flox/Cya
Common Name:
Hsp90b1-flox
Product ID:
S-CKO-06433
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hsp90b1-flox
Strain ID
CKOCMP-22027-Hsp90b1-B6J-VA
Gene Name
Product ID
S-CKO-06433
Gene Alias
ERp99; GRP94; TA-3; Targ2; Tra-1; Tra1; endoplasmin; gp96
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hsp90b1em1flox/Cya mice (Catalog S-CKO-06433) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020238
NCBI RefSeq
NM_011631
Target Region
Exon 3~4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Hsp90b1, also known as gp96 or Grp94, is a conserved member of the heat shock protein family and acts as a molecular chaperone for oncogenes, playing crucial roles in protein folding and translocation [1,3]. It is involved in multiple pathways, such as those related to tumor metabolism, cell cycle, and immune checkpoint regulation [1].
In cancer research, Hsp90b1 has been found to be highly expressed in various tumors, often correlating with poor prognosis. For example, in NSCLC patients, its overexpression is an independent poor prognostic factor for overall survival and disease-free survival [4]. In glioblastoma, it promotes the plasma membrane localization of GLUT1, enhancing glycolytic activity, tumor growth, and radioresistance [2]. In gastric cancer, it forms a positive feedback loop with LINC00501 and STAT3, promoting malignant behavior [5]. Also, the HSP90B1 inhibitor PU-WS13 can suppress cancer cell proliferation and reduce the expression of PD-L1 [1]. In the context of tuberculosis, HSP90B1 gene-region variants are associated with BCG-induced IL-2 production and may be related to protection from TB disease [6].
In conclusion, Hsp90b1 is a significant gene involved in protein-related biochemical processes. Its high expression in various cancers and association with poor prognosis, as well as its role in promoting tumor-related activities, make it a potential target for cancer diagnosis, prognosis, and therapy. In addition, its role in the immune response to Mycobacteria indicates its importance in infectious disease-related immunity [1,2,4,5,6].
References:
1. Huang, Xiaoliang, Zhang, Weiming, Yang, Na, Liu, Jungang, Zhang, Beibei. 2024. Identification of HSP90B1 in pan-cancer hallmarks to aid development of a potential therapeutic target. In Molecular cancer, 23, 19. doi:10.1186/s12943-023-01920-w. https://pubmed.ncbi.nlm.nih.gov/38243263/
2. Li, Yanhui, Ge, Yuqian, Zhao, Mengjie, Wang, Xiefeng, Qian, Xu. . HSP90B1-mediated plasma membrane localization of GLUT1 promotes radioresistance of glioblastomas. In Journal of biomedical research, 37, 326-339. doi:10.7555/JBR.37.20220234. https://pubmed.ncbi.nlm.nih.gov/37750323/
3. Wang, Yaxuan, Wang, Xiaolin. 2022. A Pan-Cancer Analysis of Heat-Shock Protein 90 Beta1(HSP90B1) in Human Tumours. In Biomolecules, 12, . doi:10.3390/biom12101377. https://pubmed.ncbi.nlm.nih.gov/36291587/
4. Xu, YiRong, Chen, ZhenWen, Zhang, GuangHeng, Chai, Fei, Li, XiongFeng. 2016. HSP90B1 overexpression predicts poor prognosis in NSCLC patients. In Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 37, 14321-14328. doi:. https://pubmed.ncbi.nlm.nih.gov/27599983/
5. Pei, Miaomiao, Xu, Nanzhu, Li, Jiaying, Lin, Jianjiao, Wang, Jide. 2023. The LINC00501-HSP90B1-STAT3 positive feedback loop promotes malignant behavior in gastric cancer cells. In Cellular signalling, 108, 110711. doi:10.1016/j.cellsig.2023.110711. https://pubmed.ncbi.nlm.nih.gov/37156452/
6. Graustein, Andrew D, Misch, Elizabeth A, Musvosvi, Munyaradzi, Scriba, Thomas J, Hawn, Thomas R. 2018. Toll-like receptor chaperone HSP90B1 and the immune response to Mycobacteria. In PloS one, 13, e0208940. doi:10.1371/journal.pone.0208940. https://pubmed.ncbi.nlm.nih.gov/30550567/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen