C57BL/6JCya-Trp53em1flox/Cya
Common Name:
Trp53-flox
Product ID:
S-CKO-06449
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Trp53-flox
Strain ID
CKOCMP-22059-Trp53-B6J-VA
Gene Name
Product ID
S-CKO-06449
Gene Alias
Tp53; bbl; bfy; bhy; p44; p53
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trp53em1flox/Cya mice (Catalog S-CKO-06449) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108658
NCBI RefSeq
NM_011640
Target Region
Exon 2~9
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Trp53, the mouse ortholog of the human TP53 tumor suppressor gene, is crucial in maintaining genomic stability. It functions in regulating the cell cycle, DNA repair, apoptosis, cellular metabolism, and senescence, acting as a "guardian of the genome" [4]. Mutations in Trp53 are associated with Li-Fraumeni syndrome, a hereditary condition characterized by early-onset tumors in multiple organs [3,4]. Genetic mouse models are valuable for studying Trp53.
Trp53-deficient mice have provided insights into its role in different biological processes. For instance, BALB/c-Trp53-deficient mice showed a delayed involution phenotype and a mammary tumor phenotype, enhancing understanding of p53's role in involution, hormonal regulation of its activity, and cooperation with other oncogenic pathways in mammary tumorigenesis [2]. In the adrenal medulla, dual loss of Rb1 and Trp53 led to spontaneous pheochromocytoma, indicating Trp53 is an efficient genetic modifier of Rb1 loss in PCC development [5]. The Trp53 +/-mouse model has been used in short-term carcinogenicity testing. It responds positively to genotoxic carcinogens, negatively to non-genotoxic rodent carcinogens and non-carcinogens, and its most common spontaneous tumors are malignant thymic lymphomas and subcutaneous sarcomas [1].
In conclusion, Trp53 is essential in multiple biological processes, with its disruption leading to various diseases, especially cancer. Mouse models, such as Trp53 knockout and conditional knockout models, have significantly contributed to understanding its role in breast cancer, pheochromocytoma development, and carcinogenicity testing, providing valuable insights into disease mechanisms and potential therapeutic strategies.
References:
1. Floyd, Eugenia, Mann, Peter, Long, Gerald, Ochoa, Ricardo. . The Trp53 hemizygous mouse in pharmaceutical development: points to consider for pathologists. In Toxicologic pathology, 30, 147-56. doi:. https://pubmed.ncbi.nlm.nih.gov/11890468/
2. Blackburn, Anneke C, Jerry, D Joseph. 2002. Knockout and transgenic mice of Trp53: what have we learned about p53 in breast cancer? In Breast cancer research : BCR, 4, 101-11. doi:. https://pubmed.ncbi.nlm.nih.gov/12052252/
3. Ossa, Carlos Andrés, Molina, Gustavo, Cock-Rada, Alicia María. 2016. Li-Fraumeni syndrome. In Biomedica : revista del Instituto Nacional de Salud, 36, 182-7. doi:10.7705/biomedica.v36i3.2793. https://pubmed.ncbi.nlm.nih.gov/27622479/
4. Merino, Diana, Malkin, David. . p53 and hereditary cancer. In Sub-cellular biochemistry, 85, 1-16. doi:10.1007/978-94-017-9211-0_1. https://pubmed.ncbi.nlm.nih.gov/25201186/
5. Tonks, Ian D, Mould, Arne W, Schroder, Wayne A, Walker, Graeme J, Kay, Graham F. . Dual loss of rb1 and Trp53 in the adrenal medulla leads to spontaneous pheochromocytoma. In Neoplasia (New York, N.Y.), 12, 235-43. doi:. https://pubmed.ncbi.nlm.nih.gov/20234817/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen