C57BL/6JCya-Trpc3em1flox/Cya
Common Name:
Trpc3-flox
Product ID:
S-CKO-06455
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Trpc3-flox
Strain ID
CKOCMP-22065-Trpc3-B6J-VA
Gene Name
Product ID
S-CKO-06455
Gene Alias
Mwk; Trcp3; Trp3; Trrp3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trpc3em1flox/Cya mice (Catalog S-CKO-06455) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029271
NCBI RefSeq
NM_019510
Target Region
Exon 2
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Trpc3, a member of the mammalian transient receptor potential (TRP) superfamily of ion channels, is a Ca2+-permeable non-selective cation channel. It is activated by various stimuli such as G-protein coupled receptors, mechanical distortion of the plasma membrane, and diacylglycerols (DAG). It participates in many physiological processes, including maintaining cellular homeostasis, and is involved in pathways related to calcium signaling. Its functions are tissue-specific, with implications in the cardiovascular system, brain, immunity, cancer, and tissue remodeling [2].
In stress-induced senescent fibroblasts, down-regulation of Trpc3 promotes cytosolic/mitochondrial Ca2+ oscillations, elevates mitochondrial Ca2+ load, mitochondrial oxygen consumption rate, and oxidative phosphorylation. Re-expression of Trpc3 in senescent cells diminishes mitochondrial Ca2+ load and promotes escape from OIS-induced senescence. Also, Trpc3 down-regulation in stromal cells leads to a pro-inflammatory and tumour-promoting secretome that encourages cancer epithelial cell proliferation and tumour growth in vivo [1]. In Trpc3-/-mice, there is defective insulin secretion and glucose intolerance, indicating its role in islet beta-cell insulin secretion [3].
In conclusion, Trpc3 plays essential roles in multiple biological processes. Model-based research, especially gene knockout mouse models, has revealed its significance in diseases such as cancer and diabetes. Understanding Trpc3 provides insights into the underlying mechanisms of these diseases and may offer potential therapeutic targets.
References:
1. Farfariello, Valerio, Gordienko, Dmitri V, Mesilmany, Lina, Lemonnier, Loïc, Prevarskaya, Natalia. 2022. TRPC3 shapes the ER-mitochondria Ca2+ transfer characterizing tumour-promoting senescence. In Nature communications, 13, 956. doi:10.1038/s41467-022-28597-x. https://pubmed.ncbi.nlm.nih.gov/35177596/
2. Tiapko, Oleksandra, Groschner, Klaus. 2018. TRPC3 as a Target of Novel Therapeutic Interventions. In Cells, 7, . doi:10.3390/cells7070083. https://pubmed.ncbi.nlm.nih.gov/30037143/
3. Rached, Gaëlle, Saliba, Youakim, Maddah, Dina, Birnbaumer, Lutz, Fares, Nassim. 2023. TRPC3 Regulates Islet Beta-Cell Insulin Secretion. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2204846. doi:10.1002/advs.202204846. https://pubmed.ncbi.nlm.nih.gov/36642838/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen