C57BL/6JCya-Tsnem1flox/Cya
Common Name
Tsn-flox
Product ID
S-CKO-06468
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22099-Tsn-B6J-VA
Status
When using this mouse strain in a publication, please cite “Tsn-flox Mouse (Catalog S-CKO-06468) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Tsn-flox
Strain ID
CKOCMP-22099-Tsn-B6J-VA
Gene Name
Product ID
S-CKO-06468
Gene Alias
2610034C24Rik, C3PO, TB-RBP
Background
C57BL/6JCya
Gene Full Name
--
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 1 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000027623
NCBI Transcript ID
NM_011650
Target Region
Exon 2~3
Size of Effective Region
~2.1 kb
Overview of Gene Research
Tsn, or Tudor-staphylococcal/micrococcal-like nuclease, is involved in miRNA decay. It promotes the degradation of miRNAs containing CA and/or UA dinucleotides, which is a process known as TumiD [1]. This function potentially impacts gene expression regulation as miRNAs are key regulators of protein-encoding transcripts.
Cellular TumiD requires the ATP-dependent RNA helicase UPF1, and UPF1 can dissociate miRNAs from their mRNA targets, making them more susceptible to TumiD. In T24 human urinary bladder cancer cells, UPF1 augments the degradation of about 50% of candidate TumiD targets. UPF1-augmented TumiD promotes the invasion of T24 cells by degrading anti-invasive miRNAs and up-regulating proinvasive proteins [1].
In conclusion, Tsn plays a crucial role in miRNA decay, which can influence gene expression. Its function in promoting the invasion of bladder cancer cells through miRNA-mediated pathways, as revealed by in vitro studies on T24 cells, provides insights into cancer-related biological processes. Understanding Tsn's role in miRNA decay may offer new perspectives on gene regulation and potential therapeutic targets in cancer.
References：
1. Elbarbary, Reyad A, Miyoshi, Keita, Hedaya, Omar, Myers, Jason R, Maquat, Lynne E. 2017. UPF1 helicase promotes TSN-mediated miRNA decay. In Genes & development, 31, 1483-1493. doi:10.1101/gad.303537.117. https://pubmed.ncbi.nlm.nih.gov/28827400/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
