C57BL/6JCya-Txn1em1flox/Cya
Common Name:
Txn1-flox
Product ID:
S-CKO-06499
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Txn1-flox
Strain ID
CKOCMP-22166-Txn1-B6J-VA
Gene Name
Product ID
S-CKO-06499
Gene Alias
ADF; Trx1; Txn
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Txn1em1flox/Cya mice (Catalog S-CKO-06499) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030051
NCBI RefSeq
NM_011660
Target Region
Exon 2~3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Txn1, encoding thioredoxin-1, is a major cellular antioxidant in mammals. It regulates the dithiol/disulfide balance of interacting proteins, playing a crucial role in the defense against oxidative stress [4,5]. Thioredoxin-1 is involved in a wide range of physiological cellular responses, and its function is closely related to intracellular redox homeostasis [4].
In the context of intracerebral hemorrhage (ICH), Txn1 was identified as a distinctively expressed RNA-binding protein. Overexpression of Txn1 in an ICH rat model reduced secondary injury and improved outcomes, and it binds to inflammation-and apoptosis-related mRNAs, affecting gene expression [1]. In a binary rat model of hypo-and hyperthyroidism, the relative expression of Txn1 increased significantly in both hypothyroid and hyperthyroid groups compared to the control group, and treatment with gallic acid decreased its expression [2]. In a study on liver fibrosis in rats, Txn1 was one of the key proteins in the combination therapy with taurine, epigallocatechin gallate and genistein [3]. In murine hematopoietic stem cells, deletion of Txn1 activated the TP53 signaling pathway and attenuated the cells' capacity to reconstitute hematopoiesis, while recombinant Txn1 promoted their proliferation and expansion [4]. In rats with a Txn1-F54L mutation, bilateral symmetrical vacuolar degeneration was observed in the midbrain, suggesting Txn1's role in reducing oxidative stress in midbrains with high energy metabolism [5].
In summary, Txn1 is essential for maintaining redox balance and is involved in multiple biological processes. Studies using various animal models, especially gene-knockout-related models, have revealed its role in diseases such as ICH, thyroid disorders, liver fibrosis, and hematopoietic stem cell regulation, as well as its importance in protecting the midbrain from oxidative stress-induced degeneration.
References:
1. Chen, Ru, Xie, Qi, Xie, Lexing, Yang, Guoqiang, Yang, Qingwu. 2024. Thioredoxin1 Binding Metastasis-Associated Lung Adenocarcinoma Transcript 1 Attenuates Inflammation and Apoptosis after Intracerebral Hemorrhage. In Aging and disease, 15, 1384-1397. doi:10.14336/AD.2023.0507. https://pubmed.ncbi.nlm.nih.gov/37196136/
2. Najafi, Zohreh, Chamani, Elham, Zarban, Asghar, Rezaei, Zohreh, Sharifzadeh, Gholamreza. 2022. The molecular evaluation of thioredoxin (TXN1 & TXN2), thioredoxin reductase 1 (TXNRd1), and oxidative stress markers in a binary rat model of hypo- and hyperthyroidism after treatment with gallic acid. In Drug and chemical toxicology, 46, 1108-1115. doi:10.1080/01480545.2022.2131812. https://pubmed.ncbi.nlm.nih.gov/36314079/
3. Cao, Wen, Li, Yan, Li, Min, Zhang, Xuerong, Liao, Ming. 2016. Txn1, Ctsd and Cdk4 are key proteins of combination therapy with taurine, epigallocatechin gallate and genistein against liver fibrosis in rats. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 85, 611-619. doi:10.1016/j.biopha.2016.11.071. https://pubmed.ncbi.nlm.nih.gov/27894668/
4. Jabbar, Shaima, Mathews, Parker, Wang, Xiaobei, Doan, Phuong L, Kang, Yubin. 2022. Thioredoxin-1 regulates self-renewal and differentiation of murine hematopoietic stem cells through p53 tumor suppressor. In Experimental hematology & oncology, 11, 83. doi:10.1186/s40164-022-00329-3. https://pubmed.ncbi.nlm.nih.gov/36316713/
5. Ohmori, Iori, Ouchida, Mamoru, Imai, Hirohiko, Toyokuni, Shinya, Mashimo, Tomoji. 2022. Thioredoxin deficiency increases oxidative stress and causes bilateral symmetrical degeneration in rat midbrain. In Neurobiology of disease, 175, 105921. doi:10.1016/j.nbd.2022.105921. https://pubmed.ncbi.nlm.nih.gov/36372289/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen