C57BL/6JCya-Cmpk2em1flox/Cya
Common Name:
Cmpk2-flox
Product ID:
S-CKO-06500
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cmpk2-flox
Strain ID
CKOCMP-22169-Cmpk2-B6J-VA
Gene Name
Product ID
S-CKO-06500
Gene Alias
1200004E04Rik; TDKI; Tyki
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cmpk2em1flox/Cya mice (Catalog S-CKO-06500) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020969
NCBI RefSeq
NM_020557
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Cmpk2, short for cytidine/uridine monophosphate kinase 2, is an essential mitochondria-associated gene. It regulates mtDNA replication and is involved in multiple pathways, such as the NLRP3 inflammasome-related inflammatory pathway [1,2,3,6,7,8]. It also has antiviral functions against various viruses including Zika virus, flaviviruses, and coronaviruses [5,9]. Its expression is associated with many biological processes and disease conditions, making it an important target for research.
In ischemic stroke, microglia/macrophage Cmpk2 knockdown using Cre recombination-dependent adeno-associated virus suppresses brain inflammatory responses, reduces infarcts, and improves neurological outcomes. It limits newly synthesized mtDNA and Ox-mtDNA formation, blocking NLRP3 inflammasome activation [1].
In spinal cord injury rats, AAV-mediated Cmpk2 knockdown or electroacupuncture-mediated Cmpk2 down-regulation inhibits NLRP3 activation, improves motor function [2].
In sepsis, dracorhodin, a Cmpk2 inhibitor, exerts anti-inflammatory effects by regulating the NLRP3 inflammasome via the LPS-induced Cmpk2 pathway [3].
In mice, homozygous Cmpk2-KO mice develop progressive calcification in the thalamus region, mimicking the brain calcification pathology in patients with biallelic CMPK2 variants [4].
In atherosclerosis mouse models, EC-GATA6 deletion decreases monocyte recruitment and atherosclerotic lesion formation through regulation of the CMPK2-Nlrp3 pathway [6].
In glucocorticoid-induced osteoporosis study, inhibiting Cmpk2 expression in preosteoblasts alleviates glucocorticoid-induced cellular senescence and promotes osteogenic differentiation [7].
In conclusion, Cmpk2 plays a crucial role in regulating mitochondrial function, inflammation, and antiviral responses. Gene knockout and conditional knockout mouse models have revealed its significant contributions in diseases such as ischemic stroke, spinal cord injury, sepsis, brain calcification, atherosclerosis, and glucocorticoid-induced osteoporosis, providing potential therapeutic targets for these conditions.
References：
1. Guan, Xin, Zhu, Sitong, Song, Jinqian, Xu, Xiaojun, Pang, Tao. 2024. Microglial CMPK2 promotes neuroinflammation and brain injury after ischemic stroke. In Cell reports. Medicine, 5, 101522. doi:10.1016/j.xcrm.2024.101522. https://pubmed.ncbi.nlm.nih.gov/38701781/
2. Chen, Yi, Wu, Lei, Shi, Mengting, Shao, XiaoMei, Ma, Ruijie. 2022. Electroacupuncture Inhibits NLRP3 Activation by Regulating CMPK2 After Spinal Cord Injury. In Frontiers in immunology, 13, 788556. doi:10.3389/fimmu.2022.788556. https://pubmed.ncbi.nlm.nih.gov/35401582/
3. Zhang, Wendan, Jiang, Honghong, Huang, Pengli, Liu, Sanhong, Zhang, Weidong. . Dracorhodin targeting CMPK2 attenuates inflammation: A novel approach to sepsis therapy. In Clinical and translational medicine, 13, e1449. doi:10.1002/ctm2.1449. https://pubmed.ncbi.nlm.nih.gov/37859535/
4. Zhao, Miao, Su, Hui-Zhen, Zeng, Yi-Heng, Cheng, Xuewen, Chen, Wan-Jin. 2022. Loss of function of CMPK2 causes mitochondria deficiency and brain calcification. In Cell discovery, 8, 128. doi:10.1038/s41421-022-00475-2. https://pubmed.ncbi.nlm.nih.gov/36443312/
5. Pawlak, Joanna B, Hsu, Jack Chun-Chieh, Xia, Hongjie, Cresswell, Peter, Laurent-Rolle, Maudry. 2023. CMPK2 restricts Zika virus replication by inhibiting viral translation. In PLoS pathogens, 19, e1011286. doi:10.1371/journal.ppat.1011286. https://pubmed.ncbi.nlm.nih.gov/37075076/
6. Wu, Wenrun, Bao, Wenzhen, Chen, Xiaoli, Zhang, Yuzhen, Zhuang, Tao. 2023. Endothelial Gata6 deletion reduces monocyte recruitment and proinflammatory macrophage formation and attenuates atherosclerosis through Cmpk2-Nlrp3 pathways. In Redox biology, 64, 102775. doi:10.1016/j.redox.2023.102775. https://pubmed.ncbi.nlm.nih.gov/37339559/
7. Cao, Nianping, Wang, Zhihang, Huang, Chongjun, Xu, Ying, Tian, Ye. 2023. Cmpk2 regulates mitochondrial function in glucocorticoid-induced osteoblast senescence and affects glucocorticoid-inhibited osteoblast differentiation. In Archives of gerontology and geriatrics, 114, 105080. doi:10.1016/j.archger.2023.105080. https://pubmed.ncbi.nlm.nih.gov/37269696/
8. Jin, Li, Chen, Qiyue, Hu, Ke, Qi, Weizhong, Yu, Qinghong. 2024. The FTO-CMPK2 Pathway in Fibroblast-like Synoviocytes Modulates Rheumatoid Arthritis Synovial Inflammation and Cartilage Homeostasis via mtDNA Regulation. In International journal of biological sciences, 20, 1617-1633. doi:10.7150/ijbs.90677. https://pubmed.ncbi.nlm.nih.gov/38481810/
9. Zhu, Mingjun, Lv, Jiahuang, Wang, Wei, Ding, Siyuan, Li, Bin. 2023. CMPK2 is a host restriction factor that inhibits infection of multiple coronaviruses in a cell-intrinsic manner. In PLoS biology, 21, e3002039. doi:10.1371/journal.pbio.3002039. https://pubmed.ncbi.nlm.nih.gov/36930652/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen