C57BL/6JCya-Ucp2em1flox/Cya
Common Name:
Ucp2-flox
Product ID:
S-CKO-06529
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ucp2-flox
Strain ID
CKOCMP-22228-Ucp2-B6J-VA
Gene Name
Product ID
S-CKO-06529
Gene Alias
Slc25a8; UCP 2; UCPH
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ucp2em1flox/Cya mice (Catalog S-CKO-06529) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000126534
NCBI RefSeq
NM_011671
Target Region
Exon 3~4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Ucp2, an uncoupling protein homolog to UCP1, is a mitochondrial anion carrier protein located in the mitochondrial inner membrane [2,3,4]. It uncouples oxidative phosphorylation from ATP production by dissipating the proton gradient across the mitochondrial inner membrane, regulating mitochondrial ATP production and the generation of reactive oxygen species (ROS) [2,4]. Ucp2 is involved in multiple pathways, such as those related to oxidative stress, cellular metabolism, cell proliferation, and cell death [3].
In endothelial cells, EC-specific Ucp2 knockout mice studies revealed that Ucp2 is a mechanosensitive gene under the control of fluid shear stress and KLF2. Ucp2 deficiency promotes atherogenesis and collagen production, while its overexpression inhibits carotid atherosclerotic plaque formation, indicating its crucial role in endothelial pro-inflammatory response and atherogenesis [1]. In podocytes, podocyte-specific Ucp2-KO mice showed that Ucp2 deficiency impairs autophagy and exacerbates podocyte injury and proteinuria in diabetic nephropathy, suggesting Ucp2 protects against hyperglycemia-induced podocyte injury by promoting autophagy [5].
In conclusion, Ucp2 plays essential roles in regulating mitochondrial function, oxidative stress, and cellular metabolism. The use of Ucp2 KO/CKO mouse models has significantly contributed to understanding its roles in diseases like atherosclerosis and diabetic nephropathy, providing insights into potential therapeutic strategies targeting Ucp2 for these conditions.
References:
1. Luo, Jiang-Yun, Cheng, Chak Kwong, He, Lei, Jo, Hanjoong, Huang, Yu. 2022. Endothelial UCP2 Is a Mechanosensitive Suppressor of Atherosclerosis. In Circulation research, 131, 424-441. doi:10.1161/CIRCRESAHA.122.321187. https://pubmed.ncbi.nlm.nih.gov/35899624/
2. Nesci, Salvatore, Rubattu, Speranza. 2024. UCP2, a Member of the Mitochondrial Uncoupling Proteins: An Overview from Physiological to Pathological Roles. In Biomedicines, 12, . doi:10.3390/biomedicines12061307. https://pubmed.ncbi.nlm.nih.gov/38927514/
3. Li, Jinran, Jiang, Rihua, Cong, Xianling, Zhao, Yunfeng. 2019. UCP2 gene polymorphisms in obesity and diabetes, and the role of UCP2 in cancer. In FEBS letters, 593, 2525-2534. doi:10.1002/1873-3468.13546. https://pubmed.ncbi.nlm.nih.gov/31330574/
4. Toda, Chitoku, Diano, Sabrina. 2014. Mitochondrial UCP2 in the central regulation of metabolism. In Best practice & research. Clinical endocrinology & metabolism, 28, 757-64. doi:10.1016/j.beem.2014.02.006. https://pubmed.ncbi.nlm.nih.gov/25256770/
5. Yang, Qianqian, Yang, Shuqing, Liang, Yuehong, Wen, Ping, Yang, Junwei. 2023. UCP2 deficiency impairs podocyte autophagy in diabetic nephropathy. In Biochimica et biophysica acta. Molecular basis of disease, 1869, 166705. doi:10.1016/j.bbadis.2023.166705. https://pubmed.ncbi.nlm.nih.gov/37023910/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen