C57BL/6NCya-Ulk1em1flox/Cya
Common Name:
Ulk1-flox
Product ID:
S-CKO-06539
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ulk1-flox
Strain ID
CKOCMP-22241-Ulk1-B6N-VA
Gene Name
Product ID
S-CKO-06539
Gene Alias
Unc51.1; mKIAA0722
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ulk1em1flox/Cya mice (Catalog S-CKO-06539) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031490
NCBI RefSeq
NM_009469
Target Region
Exon 4~5
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Ulk1, also known as unc-51 like autophagy activating kinase 1, is a serine/threonine kinase. It plays a crucial role in the autophagy pathway, integrating signals from upstream energy sensors such as mTOR and AMPK to regulate the autophagy machinery [1,4]. Ulk1 is also involved in selective autophagy processes like mitophagy, which is essential for removing damaged mitochondria [2,3].
In breast cancer, ULK1 deficiency under hypoxia attenuates mitophagy, leading to the activation of the NLRP3 inflammasome, abnormal cytokine secretion, osteoclast differentiation, and ultimately bone metastasis [2]. In diabetic nephropathy, ULK1 is identified as a potential biomarker, and its expression is associated with immune cell infiltration and GFR, potentially influencing the disease development through regulating mitophagy [3]. In pancreatic β cells, DRAK2 phosphorylates ULK1 at Ser56, inducing its ubiquitylation and suppressing autophagy, thus impairing β cell function upon overnutrition [5].
In conclusion, Ulk1 is essential for autophagy and mitophagy processes. Gene knockout-related studies in models like breast cancer, diabetic nephropathy, and pancreatic β cell function have revealed its role in these disease-related biological processes. Understanding Ulk1 can provide insights into the mechanisms of these diseases and potentially offer new therapeutic targets.
References:
1. Kim, Joungmok, Kundu, Mondira, Viollet, Benoit, Guan, Kun-Liang. 2011. AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1. In Nature cell biology, 13, 132-41. doi:10.1038/ncb2152. https://pubmed.ncbi.nlm.nih.gov/21258367/
2. Deng, Rong, Zhang, Hai-Liang, Huang, Jun-Hao, Tang, Jun, Zhu, Xiao-Feng. 2020. MAPK1/3 kinase-dependent ULK1 degradation attenuates mitophagy and promotes breast cancer bone metastasis. In Autophagy, 17, 3011-3029. doi:10.1080/15548627.2020.1850609. https://pubmed.ncbi.nlm.nih.gov/33213267/
3. Yang, Yuan-Yuan, Gao, Zhong-Xiuzi, Mao, Zi-Hui, Liu, Zhang-Suo, Wu, Peng. 2023. Identification of ULK1 as a novel mitophagy-related gene in diabetic nephropathy. In Frontiers in endocrinology, 13, 1079465. doi:10.3389/fendo.2022.1079465. https://pubmed.ncbi.nlm.nih.gov/36743936/
4. Pareek, Gautam, Kundu, Mondira. 2024. Physiological functions of ULK1/2. In Journal of molecular biology, 436, 168472. doi:10.1016/j.jmb.2024.168472. https://pubmed.ncbi.nlm.nih.gov/38311233/
5. Lu, Yuting, Xu, Junyu, Li, Yufeng, Tan, Minjia, Li, Jingya. 2024. DRAK2 suppresses autophagy by phosphorylating ULK1 at Ser56 to diminish pancreatic β cell function upon overnutrition. In Science translational medicine, 16, eade8647. doi:10.1126/scitranslmed.ade8647. https://pubmed.ncbi.nlm.nih.gov/38324636/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen