C57BL/6JCya-Utyem1flox/Cya
Common Name:
Uty-flox
Product ID:
S-CKO-06568
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Uty-flox
Strain ID
CKOCMP-22290-Uty-B6J-VA
Gene Name
Product ID
S-CKO-06568
Gene Alias
Hydb; mKIAA4057
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Y
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Utyem1flox/Cya mice (Catalog S-CKO-06568) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069309
NCBI RefSeq
NM_009484
Target Region
Exon 5
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Uty, also known as KDM6C, is a gene located on the Y chromosome. It is a homologue of KDM6A (UTX) on the X chromosome. Uty encodes a histone lysine demethylase, which is involved in removing chromatin H3K27 methylation marks at genes' transcriptional start sites for activation, participating in chromatin metabolism [4]. It is associated with various biological processes, and its study via genetic models like knockout mice can reveal its functions in vivo.
Female mice with homozygous deletion of Kdm6a do not survive, but hemizygous males are viable, suggesting Uty has a compensatory function [1]. Disruption of Uty in hematopoietic cells of male mice phenocopies the profibrotic attributes of Y chromosome loss in heart failure, indicating its role in heart failure progression [2]. In addition, knockdown of Uty in gonadectomized male mice exposed to hypoxia leads to more severe pulmonary hypertension, suggesting Uty is protective against this condition [3]. Transgenic overexpression of Uty in female mice masculinizes gene expression patterns in the placenta and hypothalamus, and affects physiological outcomes like weight gain and glucose tolerance [5].
In conclusion, Uty plays crucial roles in multiple biological processes. Gene knockout mouse models have revealed its significance in diseases such as heart failure and pulmonary hypertension. Understanding Uty's functions provides insights into the mechanisms of these diseases and potential therapeutic targets.
References:
1. Gažová, Iveta, Lengeling, Andreas, Summers, Kim M. 2019. Lysine demethylases KDM6A and UTY: The X and Y of histone demethylation. In Molecular genetics and metabolism, 127, 31-44. doi:10.1016/j.ymgme.2019.04.012. https://pubmed.ncbi.nlm.nih.gov/31097364/
2. Horitani, Keita, Chavkin, Nicholas W, Arai, Yohei, Sano, Soichi, Walsh, Kenneth. 2024. Disruption of the Uty epigenetic regulator locus in hematopoietic cells phenocopies the profibrotic attributes of Y chromosome loss in heart failure. In Nature cardiovascular research, 3, 343-355. doi:10.1038/s44161-024-00441-z. https://pubmed.ncbi.nlm.nih.gov/39183958/
3. Cunningham, Christine M, Li, Min, Ruffenach, Gregoire, Arnold, Arthur P, Eghbali, Mansoureh. . Y-Chromosome Gene, Uty, Protects Against Pulmonary Hypertension by Reducing Proinflammatory Chemokines. In American journal of respiratory and critical care medicine, 206, 186-196. doi:10.1164/rccm.202110-2309OC. https://pubmed.ncbi.nlm.nih.gov/35504005/
4. Vogt, Peter H, Zimmer, Jutta, Bender, Ulrike, Strowitzki, Thomas. 2021. AZFa candidate gene UTY and its X homologue UTX are expressed in human germ cells. In Reproduction & fertility, 2, 151-160. doi:10.1530/RAF-20-0049. https://pubmed.ncbi.nlm.nih.gov/35128450/
5. Rock, Kylie D, Folts, Lillian M, Zierden, Hannah C, Nugent, Bridget M, Bale, Tracy L. 2023. Developmental transcriptomic patterns can be altered by transgenic overexpression of Uty. In Scientific reports, 13, 21082. doi:10.1038/s41598-023-47977-x. https://pubmed.ncbi.nlm.nih.gov/38030664/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen