C57BL/6JCya-Vdac2em1flox/Cya
Common Name
Vdac2-flox
Product ID
S-CKO-06592
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22334-Vdac2-B6J-VA
When using this mouse strain in a publication, please cite “Vdac2-flox Mouse (Catalog S-CKO-06592) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Vdac2-flox
Strain ID
CKOCMP-22334-Vdac2-B6J-VA
Gene Name
Product ID
S-CKO-06592
Gene Alias
Vdac6, mVDAC2, mVDAC6
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000022293
NCBI RefSeq
NM_011695
Target Region
Exon 5~7
Size of Effective Region
~1.6 kb
Overview of Gene Research
Vdac2, or voltage-dependent anion channel 2, is a key protein in the VDAC family. It contributes to oxidative metabolism by facilitating solute transport across the outer mitochondrial membrane [7]. It is also involved in mediating local Ca(2+) transport from the sarcoplasmic reticulum to mitochondria, especially in cardiomyocytes [7]. Additionally, Vdac2 has a role in the regulation of apoptosis, interacting with the BCL-2 protein family [3].
In cancer, Vdac2 has been studied in multiple contexts. Nedd4-mediated ubiquitination of VDAC2/3 suppresses erastin-induced ferroptosis in melanoma, with Nedd4 depletion increasing cancer cell sensitivity to erastin [1]. In renal cell carcinoma (RCC), STING binds to VDAC2, and STING depletion affects mitochondrial function and RCC growth [4]. In hepatocellular carcinoma, celastrol directly targets VDAC2 to induce mitochondria-dependent cell death [5]. In lung adenocarcinoma, Hedyotis diffusa injection induces ferroptosis via the Bax/Bcl2/VDAC2/3 axis [6]. In nasopharyngeal carcinoma, TRIM21 promotes VDAC2 degradation via K48-linked ubiquitination, inhibiting mitochondrial DNA release and type-I interferon responses after radiation [8]. In sepsis-induced myocardial dysfunction, VDAC2 malonylation is involved in mitochondrial-related ferroptosis [2].
In conclusion, Vdac2 has diverse functions in metabolism, apoptosis, and Ca(2+) transport. Its role in multiple diseases, especially cancer, has been illuminated through various studies. Understanding Vdac2's functions can potentially provide new therapeutic strategies for treating cancer, sepsis-induced myocardial dysfunction, and other related diseases.
References：
1. Yang, Yongfei, Luo, Meiying, Zhang, Kexin, Shang, Yuxue, Chen, Wei. 2020. Nedd4 ubiquitylates VDAC2/3 to suppress erastin-induced ferroptosis in melanoma. In Nature communications, 11, 433. doi:10.1038/s41467-020-14324-x. https://pubmed.ncbi.nlm.nih.gov/31974380/
2. She, Han, Tan, Lei, Du, Yuanlin, Liu, Liangming, Li, Tao. 2023. VDAC2 malonylation participates in sepsis-induced myocardial dysfunction via mitochondrial-related ferroptosis. In International journal of biological sciences, 19, 3143-3158. doi:10.7150/ijbs.84613. https://pubmed.ncbi.nlm.nih.gov/37416771/
3. Yuan, Zheng, Dewson, Grant, Czabotar, Peter E, Birkinshaw, Richard W. . VDAC2 and the BCL-2 family of proteins. In Biochemical Society transactions, 49, 2787-2795. doi:10.1042/BST20210753. https://pubmed.ncbi.nlm.nih.gov/34913469/
4. Zhu, Zhichuan, Zhou, Xin, Du, Hongwei, Dotti, Gianpietro, Liu, Pengda. 2022. STING Suppresses Mitochondrial VDAC2 to Govern RCC Growth Independent of Innate Immunity. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2203718. doi:10.1002/advs.202203718. https://pubmed.ncbi.nlm.nih.gov/36445063/
5. Luo, Piao, Zhang, Qian, Shen, Shuo, Wang, Jigang, Du, Qingfeng. 2023. Mechanistic engineering of celastrol liposomes induces ferroptosis and apoptosis by directly targeting VDAC2 in hepatocellular carcinoma. In Asian journal of pharmaceutical sciences, 18, 100874. doi:10.1016/j.ajps.2023.100874. https://pubmed.ncbi.nlm.nih.gov/38149060/
6. Huang, Fuhao, Pang, Jinlong, Xu, Liansong, Li, Shanshan, Li, Xian. 2022. Hedyotis diffusa injection induces ferroptosis via the Bax/Bcl2/VDAC2/3 axis in lung adenocarcinoma. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 104, 154319. doi:10.1016/j.phymed.2022.154319. https://pubmed.ncbi.nlm.nih.gov/35853302/
7. Naghdi, Shamim, Hajnóczky, György. 2016. VDAC2-specific cellular functions and the underlying structure. In Biochimica et biophysica acta, 1863, 2503-14. doi:10.1016/j.bbamcr.2016.04.020. https://pubmed.ncbi.nlm.nih.gov/27116927/
8. Li, Jun-Yan, Zhao, Yin, Gong, Sha, Ma, Jun, Liu, Na. 2023. TRIM21 inhibits irradiation-induced mitochondrial DNA release and impairs antitumour immunity in nasopharyngeal carcinoma tumour models. In Nature communications, 14, 865. doi:10.1038/s41467-023-36523-y. https://pubmed.ncbi.nlm.nih.gov/36797289/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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