C57BL/6JCya-Kcnk9em1flox/Cya
Common Name
Kcnk9-flox
Product ID
S-CKO-06622
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-223604-Kcnk9-B6J-VA
When using this mouse strain in a publication, please cite “Kcnk9-flox Mouse (Catalog S-CKO-06622) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Kcnk9-flox
Strain ID
CKOCMP-223604-Kcnk9-B6J-VA
Gene Name
Product ID
S-CKO-06622
Gene Alias
Task3, A930009L07Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 15
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000044624
NCBI RefSeq
NM_001033876
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Overview of Gene Research
KCNK9, also known as TASK3, encodes a member of the two pore-domain potassium channel (K2P) subfamily. It regulates intracellular calcium concentration, thus modulating cell survival and inflammatory signaling pathways. It is involved in genomic imprinting, with the gene normally imprinted with paternal silencing [3].
KCNK9 deficiency in Kcnk9 -/- mice led to aggravated dextran sulfate sodium (DSS)-induced colitis, as intestinal epithelial cells showed increased intracellular calcium levels, more mitochondrial damage, and caspase-9-dependent apoptosis. KCNK9 enhances mitochondrial stability, reduces apoptosis, and supports epithelial cell survival after DSS exposure in vivo and in vitro. Also, in colon cancer, KCNK9 was overexpressed, and its down-regulation or the application of genistein could suppress cell proliferation, migration, invasion, induce cell cycle quiescence, promote apoptosis, and reduce epithelial-mesenchymal transition. Genistein could inhibit KCNK9 expression, attenuating the Wnt/β-catenin signaling pathway [2,1].
In conclusion, KCNK9 plays a crucial role in maintaining cell survival, especially in intestinal epithelial cells, by regulating calcium levels and apoptosis. In disease conditions, such as colon cancer and colitis, its function is dysregulated. Studies using Kcnk9 -/- mouse models have revealed its significance in these disease areas, providing insights into potential therapeutic strategies.
References：
1. Cheng, Yuan, Tang, Yi, Tan, Yiming, Li, Juan, Zhang, Xuping. 2023. KCNK9 mediates the inhibitory effects of genistein on hepatic metastasis from colon cancer. In Clinics (Sao Paulo, Brazil), 78, 100141. doi:10.1016/j.clinsp.2022.100141. https://pubmed.ncbi.nlm.nih.gov/36905879/
2. Pfeuffer, Steffen, Müntefering, Thomas, Rolfes, Leoni, Lüdde, Tom, Meuth, Sven G. 2022. Deficiency of the Two-Pore Potassium Channel KCNK9 Impairs Intestinal Epithelial Cell Survival and Aggravates Dextran Sodium Sulfate-Induced Colitis. In Cellular and molecular gastroenterology and hepatology, 14, 1199-1211. doi:10.1016/j.jcmgh.2022.08.003. https://pubmed.ncbi.nlm.nih.gov/35973573/
3. Graham, John M, Zadeh, Neda, Kelley, Melissa, Sagi-Dain, Lena, Shalev, Stavit A. 2016. KCNK9 imprinting syndrome-further delineation of a possible treatable disorder. In American journal of medical genetics. Part A, 170, 2632-7. doi:10.1002/ajmg.a.37740. https://pubmed.ncbi.nlm.nih.gov/27151206/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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