C57BL/6NCya-Tomm22em1flox/Cya
Common Name:
Tomm22-flox
Product ID:
S-CKO-06641
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tomm22-flox
Strain ID
CKOCMP-223696-Tomm22-B6N-VA
Gene Name
Product ID
S-CKO-06641
Gene Alias
2310047D01; Tom22
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Tomm22em1flox/Cya mice (Catalog S-CKO-06641) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023062
NCBI RefSeq
NM_172609
Target Region
Exon 1~2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
TOMM22, the translocase of outer mitochondrial membrane 22, is the central receptor of the TOMM complex. It plays a crucial role in the recognition and translocation of mitochondrial precursor proteins, and is involved in TOMM complex assembly. It is essential for maintaining mitochondrial function as most mitochondrial proteins are nuclear-encoded and imported by the TOMM complex [2,5]. Genetic models, such as zebrafish mutants and knockdown models, have been valuable in studying its function [3,6].
In a skeletal muscle-specific Csnk2b/Ck2β-conditional knockout (cKO) mouse model, reduced phosphorylation of TOMM22 was observed. This led to abnormal mitochondrial function, with reduced muscle strength and abnormal metabolic activity in oxidative muscle fibers. CSNK2-mediated phosphorylation of TOMM22 changes its binding affinity for mitochondrial precursor proteins. Additionally, PINK1 accumulated, labeling abnormal mitochondria for mitophagy. Mitophagy could be normalized by introducing a phosphomimetic TOMM22 mutant [5]. In zebrafish, knockdown of tomm22 induced hepatocyte death. This model also showed that BECs contributed to hepatocytes and surviving hepatocytes became hybrid cells, and both the inhibition of Wnt/β-catenin signaling and macrophage ablation suppressed the formation of hybrid hepatocytes [3].
In conclusion, TOMM22 is vital for mitochondrial protein import and maintaining mitochondrial homeostasis. The CKO mouse model and zebrafish knockdown models have provided insights into its role in muscle and liver-related biological processes. These findings may contribute to understanding diseases related to mitochondrial dysfunction, such as statin-induced myopathy, pancreatic cancer, and potentially Parkinson's disease [1,2,4,5].
References:
1. Yang, Neil V, Rogers, Sean, Guerra, Rachel, Theusch, Elizabeth, Krauss, Ronald M. 2023. TOMM40 and TOMM22 of the Translocase Outer Mitochondrial Membrane Complex rescue statin-impaired mitochondrial dynamics, morphology, and mitophagy in skeletal myotubes. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.06.24.546411. https://pubmed.ncbi.nlm.nih.gov/37425714/
2. Haastrup, Mary Oluwadamilola, Vikramdeo, Kunwar Somesh, Anand, Shashi, Singh, Ajay Pratap, Dasgupta, Santanu. . Mitochondrial Translocase TOMM22 Is Overexpressed in Pancreatic Cancer and Promotes Aggressive Growth by Modulating Mitochondrial Protein Import and Function. In Molecular cancer research : MCR, 22, 197-208. doi:10.1158/1541-7786.MCR-23-0138. https://pubmed.ncbi.nlm.nih.gov/37878010/
3. Wu, Jianchen, Choi, Tae-Young, Shin, Donghun. 2017. tomm22 Knockdown-Mediated Hepatocyte Damages Elicit Both the Formation of Hybrid Hepatocytes and Biliary Conversion to Hepatocytes in Zebrafish Larvae. In Gene expression, 17, 237-249. doi:10.3727/105221617X695195. https://pubmed.ncbi.nlm.nih.gov/28251883/
4. Yang, Jie, Wu, Xinyu, Song, Yuning. 2023. Recent advances in novel mutation genes of Parkinson's disease. In Journal of neurology, 270, 3723-3732. doi:10.1007/s00415-023-11781-4. https://pubmed.ncbi.nlm.nih.gov/37222843/
5. Kravic, Bojana, Harbauer, Angelika B, Romanello, Vanina, Meisinger, Chris, Hashemolhosseini, Said. 2018. In mammalian skeletal muscle, phosphorylation of TOMM22 by protein kinase CSNK2/CK2 controls mitophagy. In Autophagy, 14, 311-335. doi:10.1080/15548627.2017.1403716. https://pubmed.ncbi.nlm.nih.gov/29165030/
6. Curado, Silvia, Ober, Elke A, Walsh, Susan, Koehler, Carla M, Stainier, Didier Y R. 2010. The mitochondrial import gene tomm22 is specifically required for hepatocyte survival and provides a liver regeneration model. In Disease models & mechanisms, 3, 486-95. doi:10.1242/dmm.004390. https://pubmed.ncbi.nlm.nih.gov/20483998/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen