C57BL/6JCya-Wnt5aem1flox/Cya
Common Name
Wnt5a-flox
Product ID
S-CKO-06726
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22418-Wnt5a-B6J-VA
When using this mouse strain in a publication, please cite “Wnt5a-flox Mouse (Catalog S-CKO-06726) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Wnt5a-flox
Strain ID
CKOCMP-22418-Wnt5a-B6J-VA
Gene Name
Product ID
S-CKO-06726
Gene Alias
Wnt-5a, 8030457G12Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000063465
NCBI RefSeq
NM_009524
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Overview of Gene Research
Wnt5a is a secreted glycoprotein, belonging to the non-canonical Wnt family. It binds to Frizzled (FZD) and Receptor Tyrosine Kinase-Like Orphan Receptor (ROR) family members, activating non-canonical Wnt signaling pathways. Wnt5a is involved in diverse cellular processes such as proliferation, differentiation, migration, adhesion, and polarization, and is crucial for organism development and tissue homeostasis [2,5,6,7]. Genetic models, like KO/CKO mouse models, are valuable for studying its functions.
In colorectal cancer, high Wnt5a + CD68 + /CD68 + TAMs ratio is linked to poor prognosis. Wnt5a induces macrophages to secrete IL-10 via the CaKMII-ERK1/2-STAT3 pathway, promoting M2 polarization of tumor-associated macrophages (TAMs), which in turn promotes CRC cells proliferation, migration, and invasion [1]. In breast cancer, WNT5A knockdown in HB2 mammary epithelial cells leads to EMT-like changes and increased invasiveness, while in breast cancer cells, WNT5A signaling inhibits migration and invasion through an EMT-independent mechanism by decreasing CD44 expression [3]. In ovarian cancer, downregulating Wnt5a expression suppresses cell migration and invasion abilities [4].
In summary, Wnt5a plays a significant role in multiple cellular processes and is involved in the development and progression of various cancers, including colorectal, breast, and ovarian cancers. The study of Wnt5a using KO/CKO mouse models has revealed its complex functions in these disease conditions, providing insights into potential therapeutic strategies targeting Wnt5a for cancer treatment.
References：
1. Liu, Qing, Yang, Chaogang, Wang, Shuyi, Liu, Keshu, Xiong, Bin. 2020. Wnt5a-induced M2 polarization of tumor-associated macrophages via IL-10 promotes colorectal cancer progression. In Cell communication and signaling : CCS, 18, 51. doi:10.1186/s12964-020-00557-2. https://pubmed.ncbi.nlm.nih.gov/32228612/
2. Tufail, Muhammad, Wu, Changxin. 2023. WNT5A: a double-edged sword in colorectal cancer progression. In Mutation research. Reviews in mutation research, 792, 108465. doi:10.1016/j.mrrev.2023.108465. https://pubmed.ncbi.nlm.nih.gov/37495091/
3. Prasad, Chandra Prakash, Chaurasiya, Shivendra Kumar, Guilmain, William, Andersson, Tommy. 2016. WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition. In Journal of experimental & clinical cancer research : CR, 35, 144. doi:10.1186/s13046-016-0421-0. https://pubmed.ncbi.nlm.nih.gov/27623766/
4. Zhou, Weijian, Mei, Jie, Gu, Dingyi, Wang, Huiyu, Liu, Chaoying. 2021. Wnt5a: A promising therapeutic target in ovarian cancer. In Pathology, research and practice, 219, 153348. doi:10.1016/j.prp.2021.153348. https://pubmed.ncbi.nlm.nih.gov/33540373/
5. Bueno, Maura Lima Pereira, Saad, Sara Teresinha Olalla, Roversi, Fernanda Marconi. 2022. WNT5A in tumor development and progression: A comprehensive review. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 155, 113599. doi:10.1016/j.biopha.2022.113599. https://pubmed.ncbi.nlm.nih.gov/36089446/
6. Shi, Ya-Ning, Zhu, Neng, Liu, Chan, Liao, Duan-Fang, Qin, Li. 2017. Wnt5a and its signaling pathway in angiogenesis. In Clinica chimica acta; international journal of clinical chemistry, 471, 263-269. doi:10.1016/j.cca.2017.06.017. https://pubmed.ncbi.nlm.nih.gov/28641961/
7. Bhatt, Pooja M, Malgor, Ramiro. 2014. Wnt5a: a player in the pathogenesis of atherosclerosis and other inflammatory disorders. In Atherosclerosis, 237, 155-62. doi:10.1016/j.atherosclerosis.2014.08.027. https://pubmed.ncbi.nlm.nih.gov/25240110/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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