C57BL/6JCya-Wrnem1flox/Cya
Common Name:
Wrn-flox
Product ID:
S-CKO-06735
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Wrn-flox
Strain ID
CKOCMP-22427-Wrn-B6J-VA
Gene Name
Product ID
S-CKO-06735
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Wrnem1flox/Cya mice (Catalog S-CKO-06735) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033990
NCBI RefSeq
NM_011721
Target Region
Exon 9~10
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
WRN, encoding the Werner Syndrome RecQ helicase, is a crucial gene involved in genome maintenance. It has roles in processes such as DNA replication, base excision repair, recombination, DNA damage response, and transcription [4,6]. WRN protein is not only a helicase but also an exonuclease and ATPase, playing a key role in maintaining genome stability, especially during DNA replication and telomere metabolism [4].
Cancer cells with microsatellite instability (MSI) are selectively dependent on WRN helicase for survival [1,2,3,5,7]. Inhibition of WRN traps the helicase on chromatin, leading to its degradation via the PIAS4-RNF4-p97/VCP axis in an MSI-H dependent manner [1]. WRN can also unfold cruciform DNA structures formed by TA nucleotide repeats in MSI cells, and together with DNA mismatch repair complexes, it helps prevent DNA breaks and cell lethality [3].
In conclusion, WRN is essential for genome stability and its functions are closely related to DNA-associated processes. The discovery of its synthetic lethal interaction with MSI cancers provides a promising target for cancer therapy. Studies on WRN, especially those exploring its inhibition, may offer new strategies for treating MSI-positive tumors [1,2,3,5,7].
References:
1. Rodríguez Pérez, Fernando, Natwick, Dean, Schiff, Lauren, Bashore, Charlene, Basham, Stephen. 2024. WRN inhibition leads to its chromatin-associated degradation via the PIAS4-RNF4-p97/VCP axis. In Nature communications, 15, 6059. doi:10.1038/s41467-024-50178-3. https://pubmed.ncbi.nlm.nih.gov/39025847/
2. Wainberg, Zev A. . WRN Helicase: Is There More to MSI-H than Immunotherapy? In Cancer discovery, 14, 1369-1371. doi:10.1158/2159-8290.CD-24-0771. https://pubmed.ncbi.nlm.nih.gov/39091203/
3. Mengoli, Valentina, Ceppi, Ilaria, Sanchez, Aurore, Pettazzoni, Piergiorgio, Cejka, Petr. 2022. WRN helicase and mismatch repair complexes independently and synergistically disrupt cruciform DNA structures. In The EMBO journal, 42, e111998. doi:10.15252/embj.2022111998. https://pubmed.ncbi.nlm.nih.gov/36541070/
4. Katsuya, Tomohiro, Morishita, Ryuichi. . [WRN gene]. In Nihon rinsho. Japanese journal of clinical medicine, 67, 1277-82. doi:. https://pubmed.ncbi.nlm.nih.gov/19591272/
5. van Wietmarschen, Niek, Nathan, William J, Nussenzweig, André. 2021. The WRN helicase: resolving a new target in microsatellite unstable cancers. In Current opinion in genetics & development, 71, 34-38. doi:10.1016/j.gde.2021.06.014. https://pubmed.ncbi.nlm.nih.gov/34284257/
6. Orlovetskie, Natalie, Serruya, Raphael, Abboud-Jarrous, Ghada, Jarrous, Nayef. 2016. Targeted inhibition of WRN helicase, replication stress and cancer. In Biochimica et biophysica acta. Reviews on cancer, 1867, 42-48. doi:10.1016/j.bbcan.2016.11.004. https://pubmed.ncbi.nlm.nih.gov/27902925/
7. Morales-Juarez, David A, Jackson, Stephen P. 2022. Clinical prospects of WRN inhibition as a treatment for MSI tumours. In NPJ precision oncology, 6, 85. doi:10.1038/s41698-022-00319-y. https://pubmed.ncbi.nlm.nih.gov/36379964/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen