C57BL/6JCya-Xbp1em1flox/Cya
Common Name:
Xbp1-flox
Product ID:
S-CKO-06740
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Xbp1-flox
Strain ID
CKOCMP-22433-Xbp1-B6J-VA
Gene Name
Product ID
S-CKO-06740
Gene Alias
D11Ertd39e; TREB-5; TREB5; XBP-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Xbp1em1flox/Cya mice (Catalog S-CKO-06740) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000063084
NCBI RefSeq
NM_013842
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Xbp1, also known as X-box binding protein-1, is a transcription factor and a key regulator in the unfolded protein response (UPR) pathway. The UPR is activated upon endoplasmic reticulum (ER) stress to restore ER homeostasis. Xbp1 communicates with conserved signalling components of the UPR and is essential for cell fate determination in response to ER stress [4]. Genetic models, such as knockout (KO) mouse models, are valuable for studying Xbp1's functions.
In non-alcoholic steatohepatitis (NASH), Xbp1 expression is upregulated in human liver tissues. Hepatocyte-specific Xbp1 knockout (Xbp1ΔHep) and macrophage-specific Xbp1 knockout (Xbp1ΔMf) mice show inhibited development of steatohepatitis and fibrosis compared to wild-type mice. Xbp1-deleted macrophages reduce steatohepatitis by decreasing NLRP3 expression, pro-inflammatory cytokine secretion, and preventing hepatic stellate cell activation [1]. In colorectal cancer, Xbp1 activation in tumor-associated macrophages (TAMs) promotes tumor growth and metastasis, while Xbp1 ablation inhibits TAMs' pro-tumor cytokine signature and enhances phagocytosis [2]. In MYC-driven breast cancer, Xbp1 is a synthetic lethal partner of MYC, and silencing Xbp1 selectively blocks the growth of MYC-hyperactivated cells [3].
In conclusion, Xbp1 is crucial in the UPR pathway, affecting cell fate under ER stress. Through gene knockout mouse models, it has been revealed that Xbp1 plays significant roles in diseases like NASH, colorectal cancer, and MYC-driven breast cancer. These studies help understand the mechanisms of these diseases and provide potential therapeutic targets related to Xbp1.
References:
1. Wang, Qi, Zhou, Haoming, Bu, Qingfa, Wang, Mingming, Lu, Ling. 2022. Role of XBP1 in regulating the progression of non-alcoholic steatohepatitis. In Journal of hepatology, 77, 312-325. doi:10.1016/j.jhep.2022.02.031. https://pubmed.ncbi.nlm.nih.gov/35292349/
2. Zhao, Yahui, Zhang, Weina, Huo, Miaomiao, Xu, Ningzhi, Zhu, Hongxia. 2021. XBP1 regulates the protumoral function of tumor-associated macrophages in human colorectal cancer. In Signal transduction and targeted therapy, 6, 357. doi:10.1038/s41392-021-00761-7. https://pubmed.ncbi.nlm.nih.gov/34667145/
3. Zhao, Na, Cao, Jin, Xu, Longyong, Lewis, Michael T, Chen, Xi. 2018. Pharmacological targeting of MYC-regulated IRE1/XBP1 pathway suppresses MYC-driven breast cancer. In The Journal of clinical investigation, 128, 1283-1299. doi:10.1172/JCI95873. https://pubmed.ncbi.nlm.nih.gov/29480818/
4. Chen, Shanshan, Chen, Jing, Hua, Xin, Sha, Jun, Zhu, Xiaoli. 2020. The emerging role of XBP1 in cancer. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 127, 110069. doi:10.1016/j.biopha.2020.110069. https://pubmed.ncbi.nlm.nih.gov/32294597/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen