C57BL/6NCya-Clic5em1flox/Cya
Common Name:
Clic5-flox
Product ID:
S-CKO-06781
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Clic5-flox
Strain ID
CKOCMP-224796-Clic5-B6N-VA
Gene Name
Product ID
S-CKO-06781
Gene Alias
5730531E12Rik; B330005L24; Gm322; jbg; nmf318
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Clic5em1flox/Cya mice (Catalog S-CKO-06781) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024755
NCBI RefSeq
NM_172621
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
CLIC5, or Chloride Intracellular Channel 5, belongs to the chloride intracellular channel protein family. It has been implicated in multiple physiological processes. It can exist in both soluble and integral membrane forms, with the transmembrane form showing ion-channel activity in vitro [2]. CLIC5 is involved in pathways like the canonical Wnt/β-catenin signaling pathway, and is important for myogenesis, skeletal muscle regeneration, and maintaining normal systemic energy metabolism [1,3]. Genetic models, such as KO mouse models, have been crucial in studying its functions.
Deletion of CLIC5 in skeletal muscle leads to reduced muscle weight, decreased satellite cell number and differentiation potential. In vitro, it inhibits myoblast proliferation and promotes myotube formation, functioning through a BGN-dependent activation of the canonical Wnt/β-catenin signaling pathway [1]. CLIC5 mutant mice are lean, hyperphagic, and resistant to diet-induced obesity, but show gastric hemorrhaging, immune and neurological dysfunctions, and increased susceptibility to torpor, revealing its role in maintaining normal systemic energy metabolism [3].
In conclusion, CLIC5 is a key regulator in myogenesis and muscle regeneration via the BGN-mediated Wnt/β-catenin pathway, and is also essential for normal systemic energy metabolism. Studies using CLIC5 KO mouse models have provided valuable insights into its functions in these biological processes, which could potentially contribute to understanding related disease mechanisms such as muscle regeneration disorders and metabolic diseases.
References:
1. Zhang, Xin, He, Linjuan, Wang, Liqi, Zhao, Xingbo, Yin, Jingdong. 2024. CLIC5 promotes myoblast differentiation and skeletal muscle regeneration via the BGN-mediated canonical Wnt/β-catenin signaling pathway. In Science advances, 10, eadq6795. doi:10.1126/sciadv.adq6795. https://pubmed.ncbi.nlm.nih.gov/39999205/
2. Israeli, Tal. . High-throughput analysis of CLIC5 interactants using a thermal-stability assay. In The Israel Medical Association journal : IMAJ, 25, 853-854. doi:. https://pubmed.ncbi.nlm.nih.gov/37584386/
3. Bradford, Emily M, Miller, Marian L, Prasad, Vikram, Tso, Patrick, Shull, Gary E. 2010. CLIC5 mutant mice are resistant to diet-induced obesity and exhibit gastric hemorrhaging and increased susceptibility to torpor. In American journal of physiology. Regulatory, integrative and comparative physiology, 298, R1531-42. doi:10.1152/ajpregu.00849.2009. https://pubmed.ncbi.nlm.nih.gov/20357015/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen