C57BL/6JCya-Safbem1flox/Cya
Common Name:
Safb-flox
Product ID:
S-CKO-06795
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Safb-flox
Strain ID
CKOCMP-224903-Safb-B6J-VA
Gene Name
Product ID
S-CKO-06795
Gene Alias
3110021E02Rik; 5330423C17Rik; E130307D12; HAP; HET; SAF-B1; SAFB1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Safbem1flox/Cya mice (Catalog S-CKO-06795) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000182533
NCBI RefSeq
NM_001374619
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Safb, known as scaffold attachment factor B, is a conserved RNA-binding protein essential for early mammalian development [2]. It is involved in multiple biological processes. SAFB binds to scaffold attachment region DNA elements and is also known for its RNA-binding and protein-protein interactions. It participates in gene regulation, DNA repair, mRNA and regulatory RNA processing, and interacts with chromatin-modifying complexes [4].
In mouse models, conditional deletion of Safb in adult hippocampus increases NFIB expression and oligodendrocyte formation, indicating that SAFB blocks oligodendrocyte production in murine adult hippocampal neural stem cells by enhancing Drosha-dependent cleavage of Nfib mRNA [1]. In acute myeloid leukemia (AML), perturbation of SAFB phenocopies HOXA9 knockout, decreasing AML cell proliferation, increasing differentiation and apoptosis in vitro, and prolonging survival in vivo. The HOXA9-SAFB chromatin complex represses genes associated with differentiation and apoptosis [3].
In conclusion, SAFB plays crucial roles in multiple biological processes such as neural stem cell fate determination and in maintaining AML. The use of gene knockout and conditional knockout mouse models has provided insights into SAFB's function in these processes and diseases, which helps in understanding the underlying mechanisms and potentially developing new therapeutic strategies for AML.
References:
1. Forcella, Pascal, Ifflander, Niklas, Rolando, Chiara, Bock, Thomas, Taylor, Verdon. 2024. SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA. In eLife, 13, . doi:10.7554/eLife.74940. https://pubmed.ncbi.nlm.nih.gov/38722021/
2. Cherney, Rachel E, Eberhard, Quinn E, Giri, Gilbert, Dominguez, Daniel, Calabrese, J Mauro. 2023. SAFB associates with nascent RNAs and can promote gene expression in mouse embryonic stem cells. In RNA (New York, N.Y.), 29, 1535-1556. doi:10.1261/rna.079569.122. https://pubmed.ncbi.nlm.nih.gov/37468167/
3. Agrawal-Singh, Shuchi, Bagri, Jaana, Giotopoulos, George, Whetton, Anthony D, Huntly, Brian J P. . HOXA9 forms a repressive complex with nuclear matrix-associated protein SAFB to maintain acute myeloid leukemia. In Blood, 141, 1737-1754. doi:10.1182/blood.2022016528. https://pubmed.ncbi.nlm.nih.gov/36577137/
4. Norman, Michael, Rivers, Caroline, Lee, Youn-Bok, Idris, Jalilah, Uney, James. . The increasing diversity of functions attributed to the SAFB family of RNA-/DNA-binding proteins. In The Biochemical journal, 473, 4271-4288. doi:. https://pubmed.ncbi.nlm.nih.gov/27888239/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen