C57BL/6JCya-Srsf7em1flox/Cya
Common Name:
Srsf7-flox
Product ID:
S-CKO-06804
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Srsf7-flox
Strain ID
CKOCMP-225027-Srsf7-B6J-VA
Gene Name
Product ID
S-CKO-06804
Gene Alias
35kDa; 9430065L19Rik; 9G8; NX-96; Sfrs7
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Srsf7em1flox/Cya mice (Catalog S-CKO-06804) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000063417
NCBI RefSeq
NM_146083.2
Target Region
Exon 2~5
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Srsf7, also known as serine/arginine-rich splicing factor 7, is an essential RNA-binding protein involved in multiple aspects of RNA processing, such as splicing, mRNA nuclear export, stability, and translation [5]. It is associated with various biological pathways, including those related to cell growth, death, and immune response [4,6]. Genetic models, especially KO/CKO mouse models, are valuable for studying its functions.
In senescent cells, down-regulation of Srsf7 is observed, and its knockdown in human diploid fibroblast (HDF) can induce cellular senescence through generation of MDM2 variants, suggesting its role in p53-mediated senescence [2]. In glioblastoma, Srsf7 is a novel m6A regulator, promoting cell proliferation and migration by facilitating m6A methylation near its binding sites on relevant mRNAs [1]. In colon and lung cancer cells, Srsf7 knockdown inhibits proliferation and enhances apoptosis, and it regulates the splicing of the apoptosis regulator Fas [5]. In murine diabetic kidney disease, diabetes down-regulates Srsf7 in the proximal tubule, and its in vitro knockdown induces a pro-inflammatory phenotype [3].
In conclusion, Srsf7 plays crucial roles in multiple biological processes, including cell senescence, cancer progression, and diabetic kidney disease. Studies using KO/CKO mouse models and other loss-of-function experiments have revealed its functions in these specific disease areas, providing insights into the underlying molecular mechanisms and potential therapeutic targets.
References:
1. Cun, Yixian, An, Sanqi, Zheng, Haiqing, Wang, Jinkai, Li, Jun. 2021. Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma. In Genomics, proteomics & bioinformatics, 21, 707-728. doi:10.1016/j.gpb.2021.11.001. https://pubmed.ncbi.nlm.nih.gov/34954129/
2. Hong, Jiwon, Min, Seongki, Yoon, Gyesoon, Lim, Su Bin. 2023. SRSF7 downregulation induces cellular senescence through generation of MDM2 variants. In Aging, 15, 14591-14606. doi:10.18632/aging.205420. https://pubmed.ncbi.nlm.nih.gov/38159247/
3. Wu, Haojia, Gonzalez Villalobos, Romer, Yao, Xiang, Breyer, Matthew D, Humphreys, Benjamin D. 2022. Mapping the single-cell transcriptomic response of murine diabetic kidney disease to therapies. In Cell metabolism, 34, 1064-1078.e6. doi:10.1016/j.cmet.2022.05.010. https://pubmed.ncbi.nlm.nih.gov/35709763/
4. Zhao, Lingcai, Li, Shengmin, Deng, Lulu, Xia, Jun, Ping, Jihui. 2024. Host-specific SRSF7 regulates polymerase activity and replication of influenza A virus. In Microbes and infection, 26, 105401. doi:10.1016/j.micinf.2024.105401. https://pubmed.ncbi.nlm.nih.gov/39134172/
5. Fu, Yu, Wang, Yingze. 2018. SRSF7 knockdown promotes apoptosis of colon and lung cancer cells. In Oncology letters, 15, 5545-5552. doi:10.3892/ol.2018.8072. https://pubmed.ncbi.nlm.nih.gov/29556298/
6. Shen, Wei, Yuan, Lebin, Cheng, Fei, Wu, Zhao, Li, Xiaodong. 2023. SRSF7 is a promising prognostic biomarker in hepatocellular carcinoma and is associated with immune infiltration. In Genes & genomics, 46, 49-64. doi:10.1007/s13258-023-01463-w. https://pubmed.ncbi.nlm.nih.gov/37985547/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen