C57BL/6JCya-Kmt5bem1flox/Cya
Common Name:
Kmt5b-flox
Product ID:
S-CKO-06860
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Kmt5b-flox
Strain ID
CKOCMP-225888-Kmt5b-B6J-VA
Gene Name
Product ID
S-CKO-06860
Gene Alias
C630029K18Rik; Suv4-20h1; Suv420h1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kmt5bem1flox/Cya mice (Catalog S-CKO-06860) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113973
NCBI RefSeq
NM_001167885
Target Region
Exon 5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
KMT5B, also known as lysine methyltransferase 5B or SUV4-20H1, is a lysine methyltransferase that plays a crucial role in histone modification, which is essential for regulating gene expression. This process is involved in numerous biological pathways, especially those related to nervous system development and function [1,3,6-9]. Genetic models, such as mouse models, are valuable tools for studying the function of KMT5B.
In Kmt5b homozygous knockout mice, they were smaller in size than wild-type littermates, suggesting relative macrocephaly as seen in human patients [1]. Kmt5b haploinsufficient mice also showed a skeletal muscle developmental deficit with reduced muscle mass and body weight, affecting neuromuscular strength and NMJ structure [2]. In vivo knockdown of Kmt5b in the mouse embryonic cerebral cortex led to decreased proliferation and accelerated migration of neural progenitor cells [3]. Heterozygous Kmt5b mice had deficits in neonatal reflexes, sociability, and changes in thermal pain sensing, with some outcomes differing by sex [4].
In conclusion, KMT5B is essential for normal development, particularly in the nervous system and skeletal muscle. Studies using KO mouse models have revealed its role in processes like neurogenesis, neuronal migration, and muscle development. These findings contribute to understanding KMT5B-related neurodevelopmental disorders, including global developmental delay, autism, and congenital anomalies [1,3-7,9,10].
References:
1. Sheppard, Sarah E, Bryant, Laura, Wickramasekara, Rochelle N, Bhoj, Elizabeth J, Stessman, Holly A F. 2023. Mechanism of KMT5B haploinsufficiency in neurodevelopment in humans and mice. In Science advances, 9, eade1463. doi:10.1126/sciadv.ade1463. https://pubmed.ncbi.nlm.nih.gov/36897941/
2. Hulen, Jason, Kenny, Dorothy, Black, Rebecca, Abel, Peter W, Stessman, Holly A F. 2022. KMT5B is required for early motor development. In Frontiers in genetics, 13, 901228. doi:10.3389/fgene.2022.901228. https://pubmed.ncbi.nlm.nih.gov/36035149/
3. Chen, Guodong, Han, Lin, Tan, Senwei, Xia, Kun, Guo, Hui. 2022. Loss-of-function of KMT5B leads to neurodevelopmental disorder and impairs neuronal development and neurogenesis. In Journal of genetics and genomics = Yi chuan xue bao, 49, 881-890. doi:10.1016/j.jgg.2022.03.004. https://pubmed.ncbi.nlm.nih.gov/35331928/
4. Wickramasekara, Rochelle N, Robertson, Brynn, Hulen, Jason, Hallgren, Jodi, Stessman, Holly A F. 2021. Differential effects by sex with Kmt5b loss. In Autism research : official journal of the International Society for Autism Research, 14, 1554-1571. doi:10.1002/aur.2516. https://pubmed.ncbi.nlm.nih.gov/33871180/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen