C57BL/6NCya-Atrxem1flox/Cya
Common Name:
Atrx-flox
Product ID:
S-CKO-06861
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Atrx-flox
Strain ID
CKOCMP-22589-Atrx-B6N-VA
Gene Name
Product ID
S-CKO-06861
Gene Alias
4833408C14Rik; ATR2; DXHXS6677E; HP1-BP38; Hp1bp2; Hp1bp38; MRXS3; RAD54L; Rad54; XH2; Xnp; ZNF-HX
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Atrxem1flox/Cya mice (Catalog S-CKO-06861) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113573
NCBI RefSeq
NM_009530
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
ATRX, short for alpha-thalassemia mental retardation X-linked, is a tumor suppressor gene. It belongs to the SWI-SNF family and is crucial for multiple molecular pathways, such as regulating chromatin state, gene expression, and DNA damage repair. ATRX deposits the histone variant H3.3 into repetitive heterochromatin, including telomeres, and is thus central to maintaining genome stability and function [1,3,4,6].
In glioma, ATRX mutations are common. These mutations can lead to alternative lengthening of telomeres (ALT), a telomere maintenance mechanism in some tumors. ATRX up-regulation via DNA demethylation in temozolomide (TMZ)-resistant glioma cells strengthens DNA damage repair by stabilizing PARP1 protein, which is related to TMZ resistance [2]. Also, loss of ATRX in tumors may suppress anti-tumor immunity [5].
In conclusion, ATRX is essential for maintaining genome stability through its role in chromatin regulation and telomere function. Its mutations are closely associated with glioma, affecting tumor development, treatment response, and anti-tumor immunity. Research on ATRX, especially through gene knockout or conditional knockout models, helps understand its functions in disease, potentially leading to new therapeutic strategies for glioma and other related cancers [1-10].
References：
1. Aguilera, Paula, López-Contreras, Andrés J. 2023. ATRX, a guardian of chromatin. In Trends in genetics : TIG, 39, 505-519. doi:10.1016/j.tig.2023.02.009. https://pubmed.ncbi.nlm.nih.gov/36894374/
2. Han, Bo, Meng, Xiangqi, Wu, Pengfei, Cai, Jinquan, Jiang, Tao. 2020. ATRX/EZH2 complex epigenetically regulates FADD/PARP1 axis, contributing to TMZ resistance in glioma. In Theranostics, 10, 3351-3365. doi:10.7150/thno.41219. https://pubmed.ncbi.nlm.nih.gov/32194873/
3. Dyer, Michael A, Qadeer, Zulekha A, Valle-Garcia, David, Bernstein, Emily. 2017. ATRX and DAXX: Mechanisms and Mutations. In Cold Spring Harbor perspectives in medicine, 7, . doi:10.1101/cshperspect.a026567. https://pubmed.ncbi.nlm.nih.gov/28062559/
4. Valenzuela, Martina, Amato, Roberta, Sgura, Antonella, Antoccia, Antonio, Berardinelli, Francesco. 2021. The Multiple Facets of ATRX Protein. In Cancers, 13, . doi:10.3390/cancers13092211. https://pubmed.ncbi.nlm.nih.gov/34062956/
5. Diaz, Aaron A. . Loss of ATRX suppresses anti-tumor immunity. In Neuro-oncology, 24, 901-902. doi:10.1093/neuonc/noac059. https://pubmed.ncbi.nlm.nih.gov/35235678/
6. Pang, Ying, Chen, Xu, Ji, Tongjie, Zhang, Jing, Zhong, Chunlong. 2023. The Chromatin Remodeler ATRX: Role and Mechanism in Biology and Cancer. In Cancers, 15, . doi:10.3390/cancers15082228. https://pubmed.ncbi.nlm.nih.gov/37190157/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen