C57BL/6JCya-Myrfem1flox/Cya
Common Name:
Myrf-flox
Product ID:
S-CKO-06866
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Myrf-flox
Strain ID
CKOCMP-225908-Myrf-B6J-VA
Gene Name
Product ID
S-CKO-06866
Gene Alias
6030439E18; Gm1804; Gm98; Mrf
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Myrfem1flox/Cya mice (Catalog S-CKO-06866) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000189897
NCBI RefSeq
NM_001033481
Target Region
Exon 8
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Myrf, short for Myelin Regulator Factor, is a master regulator governing myelin formation and maintenance in the central nervous system [2]. It is an unconventional transmembrane transcription factor that undergoes proteolytic self-processing for transcriptional regulation. Myrf is conserved across metazoans and has broad tissue expression, suggesting functions beyond myelination [2].
Microglia-derived exosomes can transfer miR-615-5p to oligodendrocyte precursor cells (OPCs), where miR-615-5p binds to MYRF and inhibits OPC maturation, thus modulating myelin regeneration. Targeting the miR-615-5p/MYRF axis could be a new therapy for demyelinating diseases [1]. In Myrfmut/+ mice, there are elevated intraocular pressure, fewer ganglion cells, and a thinner retinal nerve fiber layer, along with down-regulation of Dnmt3a, suggesting MYRF mutations are associated with primary angle-closure glaucoma [4]. Nanophthalmos-associated MYRF mutation in mice makes the eyes more susceptible to inflammation, which can be relieved by dexamethasone treatment [5]. Heterozygous loss-of-function variants in MYRF in humans can lead to abnormal development of the heart, genitourinary tract, diaphragm, and lungs, with phenotypes including congenital heart defects, genitourinary anomalies, congenital diaphragmatic hernia, and pulmonary hypoplasia [3]. MYRF haploinsufficiency also causes 46,XY and 46,XX disorders of sex development, with clinical symptoms like hypoplasia of Müllerian derivatives and ovaries in 46,XX DSD patients, and defective development of Sertoli and Leydig cells in 46,XY DSD patients [6].
In conclusion, Myrf plays crucial roles in myelin formation, and its mutations are associated with various diseases such as demyelinating diseases, primary angle-closure glaucoma, intraocular inflammation, and disorders of sex development. Studies on Myrf-related KO or mutant mouse models have provided valuable insights into these disease mechanisms, facilitating a better understanding of the gene's functions and potential therapeutic targets.
References:
1. Ji, Xiao-Yu, Guo, Yu-Xin, Wang, Li-Bin, Zhang, Yuan, Li, Xing. 2024. Microglia-derived exosomes modulate myelin regeneration via miR-615-5p/MYRF axis. In Journal of neuroinflammation, 21, 29. doi:10.1186/s12974-024-03019-5. https://pubmed.ncbi.nlm.nih.gov/38246987/
2. Qi, Yingchuan B, Xu, Zhimin, Shen, Shiqian, Wang, Zhao, Wang, Zhizhi. 2024. MYRF: A unique transmembrane transcription factor- from proteolytic self-processing to its multifaceted roles in animal development. In BioEssays : news and reviews in molecular, cellular and developmental biology, 46, e2300209. doi:10.1002/bies.202300209. https://pubmed.ncbi.nlm.nih.gov/38488284/
3. Rossetti, Linda Z, Glinton, Kevin, Yuan, Bo, Lalani, Seema R, Scott, Daryl A. 2019. Review of the phenotypic spectrum associated with haploinsufficiency of MYRF. In American journal of medical genetics. Part A, 179, 1376-1382. doi:10.1002/ajmg.a.61182. https://pubmed.ncbi.nlm.nih.gov/31069960/
4. Ouyang, Jiamin, Sun, Wenmin, Shen, Huangxuan, Tan, Zhiqun, Zhang, Qingjiong. 2022. Truncation mutations in MYRF underlie primary angle closure glaucoma. In Human genetics, 142, 103-123. doi:10.1007/s00439-022-02487-0. https://pubmed.ncbi.nlm.nih.gov/36129575/
5. Yu, Xiaowei, Zhang, Miao, Zhao, Hanxue, Shi, Yan, Fan, Zhigang. 2024. Nanophthalmos-Associated MYRF gene mutation facilitates intraocular inflammation in mice. In International immunopharmacology, 137, 112519. doi:10.1016/j.intimp.2024.112519. https://pubmed.ncbi.nlm.nih.gov/38901241/
6. Hamanaka, Kohei, Takata, Atsushi, Uchiyama, Yuri, Ogata, Tsutomu, Matsumoto, Naomichi. . MYRF haploinsufficiency causes 46,XY and 46,XX disorders of sex development: bioinformatics consideration. In Human molecular genetics, 28, 2319-2329. doi:10.1093/hmg/ddz066. https://pubmed.ncbi.nlm.nih.gov/30985895/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen