C57BL/6JCya-Nmrk1em1flox/Cya
Common Name:
Nmrk1-flox
Product ID:
S-CKO-06878
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Nmrk1-flox
Strain ID
CKOCMP-225994-Nmrk1-B6J-VA
Gene Name
Product ID
S-CKO-06878
Gene Alias
D630020N23Rik; Nrk1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nmrk1em1flox/Cya mice (Catalog S-CKO-06878) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000237347
NCBI RefSeq
NM_145497
Target Region
Exon 3~4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Nmrk1, also known as nicotinamide riboside kinase 1, is an essential enzyme in the utilization of nicotinamide riboside (NR), playing a crucial role in maintaining NAD+ levels [3]. NAD+ is a co-enzyme in redox reactions and a substrate for various enzyme families, and thus Nmrk1 is involved in pathways related to energy metabolism, such as glycolipid metabolism [1,2].
In whole-body and pancreatic β-cell-specific Nrk1 knockout (KO) mice, Nrk1 deficiency led to glucose intolerance and compromised β-cell response to a glucose challenge upon high-fat feeding or aging, indicating its role in maintaining pancreatic β-cell function and glucose tolerance [3]. In liver-specific Nrk1 KO mice, it resulted in decreased gluconeogenic potential, impaired mitochondrial function, and more susceptibility to diet-induced liver DNA damage, suggesting its importance in sustaining hepatic NAD+ levels and hindering diet-induced metabolic damage [4].
In conclusion, Nmrk1 is vital for maintaining NAD+ levels, which is essential for normal function in processes like energy metabolism and glucose homeostasis. Studies using Nrk1 KO mouse models have revealed its significance in pancreatic β-cell function and hepatic metabolism, highlighting its potential as a therapeutic target for metabolic disorders such as type 2 diabetes and hepatic steatosis [1,3,4].
References:
1. Fan, Rui, Cui, Jing, Ren, Feng, Qian, Xinlai, Xiong, Xiwen. . Overexpression of NRK1 ameliorates diet- and age-induced hepatic steatosis and insulin resistance. In Biochemical and biophysical research communications, 500, 476-483. doi:10.1016/j.bbrc.2018.04.107. https://pubmed.ncbi.nlm.nih.gov/29678570/
2. Zhang, Jinqi, Xing, Ya, Li, Fangbo, Gong, Daoqing, Geng, Tuoyu. 2023. Study on the Mechanism of MC5R Participating in Energy Metabolism of Goose Liver. In International journal of molecular sciences, 24, . doi:10.3390/ijms24108648. https://pubmed.ncbi.nlm.nih.gov/37239994/
3. Cercillieux, Angelique, Ratajczak, Joanna, Joffraud, Magali, Sambeat, Audrey, Canto, Carles. 2022. Nicotinamide riboside kinase 1 protects against diet and age-induced pancreatic β-cell failure. In Molecular metabolism, 66, 101605. doi:10.1016/j.molmet.2022.101605. https://pubmed.ncbi.nlm.nih.gov/36165811/
4. Sambeat, Audrey, Ratajczak, Joanna, Joffraud, Magali, Moco, Sofia, Canto, Carles. 2019. Endogenous nicotinamide riboside metabolism protects against diet-induced liver damage. In Nature communications, 10, 4291. doi:10.1038/s41467-019-12262-x. https://pubmed.ncbi.nlm.nih.gov/31541116/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen