C57BL/6JCya-Yap1em1flox/Cya
Common Name:
Yap1-flox
Product ID:
S-CKO-06882
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Yap1-flox
Strain ID
CKOCMP-22601-Yap1-B6J-VA
Gene Name
Product ID
S-CKO-06882
Gene Alias
Yap; Yap65; Yki; Yorkie
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Yap1em1flox/Cya mice (Catalog S-CKO-06882) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000065353
NCBI RefSeq
NM_001171147
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Yap1, or Yes-associated protein 1, is a transcriptional co-activator. Its activity is mainly regulated by the Hippo signaling pathway. It plays crucial roles in normal tissue homeostasis, regeneration, and is also involved in numerous biological processes such as cell proliferation, survival, and differentiation [3].
In immune thrombocytopenia (ITP), reduced Yap1 expression in megakaryocytes (MKs) was observed. In an ITP mouse model, decreased Yap1 led to aberrant MK distribution, reduced late MK percentage, and submaximal platelet recovery. Mechanistically, GATA-binding protein 1 binding to the Yap1 promoter upregulated Yap1, promoting MK maturation, and phosphorylated Yap1 facilitated thrombopoiesis by binding to myosin heavy chain 9 [1].
In diabetic kidney disease (DKD), bioinformatic analysis showed Yap1 inactivation and MQC dysregulation in renal tubule cells. Tubule cell-specific Yap1 deletion in diabetic mice exacerbated kidney injury, disrupting MQC, and promoting M1 macrophage infiltration. Administering the Yap1 activator XMU-MP-1 ameliorated these effects [2].
In summary, Yap1 is essential for normal physiological processes. Its dysregulation is implicated in diseases like ITP and DKD. Gene knockout or conditional knockout mouse models have been instrumental in uncovering its role in these disease conditions, highlighting its potential as a therapeutic target.
References:
1. Hu, Shuhong, Liu, Yifei, Zhang, Xiang, Wu, Depei, Xu, Yang. . YAP1 regulates thrombopoiesis by binding to MYH9 in immune thrombocytopenia. In Blood, 144, 2136-2148. doi:10.1182/blood.2023023601. https://pubmed.ncbi.nlm.nih.gov/39190466/
2. Ye, Siyang, Zhang, Meng, Zheng, Xunhua, Li, Bin, Chen, Wei. 2024. YAP1 preserves tubular mitochondrial quality control to mitigate diabetic kidney disease. In Redox biology, 78, 103435. doi:10.1016/j.redox.2024.103435. https://pubmed.ncbi.nlm.nih.gov/39608245/
3. Szulzewsky, Frank, Holland, Eric C, Vasioukhin, Valeri. 2021. YAP1 and its fusion proteins in cancer initiation, progression and therapeutic resistance. In Developmental biology, 475, 205-221. doi:10.1016/j.ydbio.2020.12.018. https://pubmed.ncbi.nlm.nih.gov/33428889/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen