C57BL/6JCya-Trpm3em1flox/Cya
Common Name:
Trpm3-flox
Product ID:
S-CKO-06885
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Trpm3-flox
Strain ID
CKOCMP-226025-Trpm3-B6J-VB
Gene Name
Product ID
S-CKO-06885
Gene Alias
6330504P12Rik; 9330180E14; B930001P07Rik; LTRPC3; MLSN2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trpm3em1flox/Cya mice (Catalog S-CKO-06885) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000237820
NCBI RefSeq
NM_001035239
Target Region
Exon 4
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Trpm3, a member of the TRP family, encodes proteins forming cation-permeable ion channels on the plasma membrane [1]. It has unique features like generating numerous isoforms mainly through alternative splicing. An intronic microRNA (miR-204) co-transcribed with Trpm3 increases its functional complexity. TRPM3 channels are involved in pathways such as sensing noxious heat, modulating insulin release, and cytokine secretion, and are expressed in various tissues like nociceptive neurons, pancreatic beta cells, and vascular smooth muscle cells [1]. Adequate pharmacological and genetic tools make it possible to study Trpm3 further.
In in vivo behavioral studies using an acute oxaliplatin model for chemotherapy-induced peripheral neuropathic pain (CIPNP), control mice developed cold and mechano-hypersensitivity, while TRPM3 -deficient mice did not. Also, the level of protein ERK, a neuronal activity marker, was significantly reduced in dorsal root ganglion neurons from TRPM3 -deficient mice after oxaliplatin administration. Intraperitoneal injection of a TRPM3 antagonist effectively reduced oxaliplatin-induced pain behavior, suggesting TRPM3 as a potential target for treating neuropathic pain in chemotherapy patients [2].
In conclusion, Trpm3 is crucial for functions like thermosensation, pain transduction, and regulation of insulin and cytokine secretion. The study of TRPM3 -deficient mouse models has revealed its potential role in chemotherapy-induced peripheral neuropathic pain, offering new insights into the treatment of this condition.
References:
1. Oberwinkler, Johannes, Philipp, Stephan E. . TRPM3. In Handbook of experimental pharmacology, 222, 427-59. doi:10.1007/978-3-642-54215-2_17. https://pubmed.ncbi.nlm.nih.gov/24756716/
2. Aloi, Vincenzo Davide, Pinto, Sílvia João Poseiro Coutinho, Van Bree, Rita, Voets, Thomas, Vriens, Joris. 2023. TRPM3 as a novel target to alleviate acute oxaliplatin-induced peripheral neuropathic pain. In Pain, 164, 2060-2069. doi:10.1097/j.pain.0000000000002906. https://pubmed.ncbi.nlm.nih.gov/37079852/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen