C57BL/6JCya-Zap70em1flox/Cya
Common Name
Zap70-flox
Product ID
S-CKO-06933
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22637-Zap70-B6J-VA
Status
When using this mouse strain in a publication, please cite “Zap70-flox Mouse (Catalog S-CKO-06933) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Zap70-flox
Strain ID
CKOCMP-22637-Zap70-B6J-VA
Gene Name
Product ID
S-CKO-06933
Gene Alias
Srk, mur, mrtle, ZAP-70
Background
C57BL/6JCya
Gene Full Name
zeta-chain (TCR) associated protein kinase
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 1 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000027291
NCBI Transcript ID
NM_009539
Target Region
Exon 4~13
Size of Effective Region
~4.9 kb
Overview of Gene Research
Zap70, short for zeta-chain associated protein kinase 70 kDa, is a nonreceptor tyrosine kinase. It plays essential roles in initiating T-cell receptor (TCR) signaling in T-lymphocytes, being at the crossroads of several signaling pathways that control T lymphocyte development and function [2]. It is also involved in B-cell receptor (BCR) signaling in certain B-cell malignancies. The PI3K-pathway is one of the associated pathways, and its normal developmental separation from SYK is crucial as a safeguard [1].
Mouse models have revealed that partial defects in Zap70 activity can be associated with autoimmunity, suggesting its involvement in the balance between immunity and tolerance [2]. In B-cell chronic lymphocytic leukemia (CLL), aberrant co-expression of Zap70 with SYK is considered a biomarker of aggressive disease and poor clinical outcomes. Mouse models may help further understand how this aberrant expression functions as an oncogenic driver in CLL and other B-cell malignancies [1].
In conclusion, Zap70 is a key regulator in adaptive immunity, with its function being essential for normal T-cell development and function. Its abnormal expression is linked to diseases like autoimmunity and B-cell malignancies. Studies using mouse models have been instrumental in uncovering its role in maintaining the balance between immunity and tolerance and in understanding disease-associated mechanisms.
References：
1. Leveille, Etienne, Chan, Lai N, Mirza, Abu-Sayeef, Kume, Kohei, Müschen, Markus. 2022. SYK and ZAP70 kinases in autoimmunity and lymphoid malignancies. In Cellular signalling, 94, 110331. doi:10.1016/j.cellsig.2022.110331. https://pubmed.ncbi.nlm.nih.gov/35398488/
2. Fischer, Alain, Picard, Capucine, Chemin, Karine, le Deist, Françoise, Hivroz, Claire. 2010. ZAP70: a master regulator of adaptive immunity. In Seminars in immunopathology, 32, 107-16. doi:10.1007/s00281-010-0196-x. https://pubmed.ncbi.nlm.nih.gov/20135127/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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