C57BL/6JCya-Rnf103em1flox/Cya
Common Name
Rnf103-flox
Product ID
S-CKO-06946
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22644-Rnf103-B6J-VA
Status
When using this mouse strain in a publication, please cite “Rnf103-flox Mouse (Catalog S-CKO-06946) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Rnf103-flox
Strain ID
CKOCMP-22644-Rnf103-B6J-VA
Gene Name
Product ID
S-CKO-06946
Gene Alias
kf-1, Zfp103
Background
C57BL/6JCya
Gene Full Name
ring finger protein 103
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 6 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000064637
NCBI Transcript ID
NM_009543
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Overview of Gene Research
Rnf103, also known as ring finger protein 103, is an E3 ubiquitin-protein ligase. E3 ubiquitin-protein ligases play a crucial role in the ubiquitination process, which is involved in many biological pathways such as protein degradation, cell cycle regulation, and immune response [1,4].
In aquaculture, Rnf103 has been identified as a key target in Vibrio anguillarum immune evasion. It promotes immune escape by inhibiting Traf6. A circular RNA, circRnf103, formed by reverse splicing of the Rnf103 gene, encodes Rnf103-177aa. This protein competes with Rnf103 and binds to Traf6, preventing its degradation. circRnf103 therapy is effective in treating V. anguillarum infections in zebrafish models [1].
In diabetic foot ulcers (DFU), RNF103-CHMP3 (a form related to Rnf103) was identified as a key gene significantly associated with DFU, linked to extracellular interactions and potentially involved in cellular communication and tissue repair mechanisms for wound healing [2].
In Alzheimer's disease, Rnf103 was among six characteristic genes determined by machine-learning algorithms, enabling the prediction of AD progression [3].
In pigs, Rnf103 was considered as a genetic marker for growth traits [5]. In a study on blood lipid levels, Rnf103 was associated with triglycerides [6].
In conclusion, Rnf103 is an important E3 ubiquitin-protein ligase involved in multiple biological processes and diseases. Studies in different models, such as zebrafish for Vibrio infection, have revealed its role in immune-related processes. In diseases like DFU, Alzheimer's, and in relation to lipid metabolism and pig growth, Rnf103 shows potential as a key gene, highlighting its significance in understanding disease mechanisms and potentially developing new treatments.
References：
1. Zheng, Weiwei, Lv, Xing, Tao, Yaqi, Zhu, Tongtong, Xu, Tianjun. 2023. A circRNA therapy based on Rnf103 to inhibit Vibrio anguillarum infection. In Cell reports, 42, 113314. doi:10.1016/j.celrep.2023.113314. https://pubmed.ncbi.nlm.nih.gov/37874674/
2. Yu, Xin, Wu, Zhuo, Zhang, Nan. 2024. Machine learning-driven discovery of novel therapeutic targets in diabetic foot ulcers. In Molecular medicine (Cambridge, Mass.), 30, 215. doi:10.1186/s10020-024-00955-z. https://pubmed.ncbi.nlm.nih.gov/39543487/
3. Lai, Yongxing, Lin, Xueyan, Lin, Chunjin, Chen, Zhihan, Zhang, Li. 2022. Identification of endoplasmic reticulum stress-associated genes and subtypes for prediction of Alzheimer's disease based on interpretable machine learning. In Frontiers in pharmacology, 13, 975774. doi:10.3389/fphar.2022.975774. https://pubmed.ncbi.nlm.nih.gov/36059957/
4. Scheper, Johanna, Oliva, Baldo, Villà-Freixa, Jordi, Thomson, Timothy M. . Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes. In Proteins, 74, 92-103. doi:10.1002/prot.22120. https://pubmed.ncbi.nlm.nih.gov/18615712/
5. Li, Xiaoping, Kim, Sang-Wook, Do, Kyoung-Tag, Choi, Bong-Hwan, Kim, Kwan-Suk. 2010. Analyses of porcine public SNPs in coding-gene regions by re-sequencing and phenotypic association studies. In Molecular biology reports, 38, 3805-20. doi:10.1007/s11033-010-0496-1. https://pubmed.ncbi.nlm.nih.gov/21107721/
6. Li, Changwei, Bazzano, Lydia A L, Rao, Dabeeru C, Lu, Xiangfeng, Kelly, Tanika N. 2015. Genome-wide linkage and positional association analyses identify associations of novel AFF3 and NTM genes with triglycerides: the GenSalt study. In Journal of genetics and genomics = Yi chuan xue bao, 42, 107-17. doi:10.1016/j.jgg.2015.02.003. https://pubmed.ncbi.nlm.nih.gov/25819087/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
