C57BL/6JCya-Rnf103em1flox/Cya
Common Name
Rnf103-flox
Product ID
S-CKO-06946
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22644-Rnf103-B6J-VA
When using this mouse strain in a publication, please cite “Rnf103-flox Mouse (Catalog S-CKO-06946) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Rnf103-flox
Strain ID
CKOCMP-22644-Rnf103-B6J-VA
Gene Name
Product ID
S-CKO-06946
Gene Alias
Zfp103, kf-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000064637
NCBI RefSeq
NM_009543
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Overview of Gene Research
Rnf103, also known as ring finger protein 103, is an E3 ubiquitin-protein ligase. E3 ubiquitin-protein ligases play a crucial role in the ubiquitination process, which is involved in many biological pathways such as protein degradation, cell cycle regulation, and immune response [1,4].
In aquaculture, Rnf103 has been identified as a key target in Vibrio anguillarum immune evasion. It promotes immune escape by inhibiting Traf6. A circular RNA, circRnf103, formed by reverse splicing of the Rnf103 gene, encodes Rnf103-177aa. This protein competes with Rnf103 and binds to Traf6, preventing its degradation. circRnf103 therapy is effective in treating V. anguillarum infections in zebrafish models [1].
In diabetic foot ulcers (DFU), RNF103-CHMP3 (a form related to Rnf103) was identified as a key gene significantly associated with DFU, linked to extracellular interactions and potentially involved in cellular communication and tissue repair mechanisms for wound healing [2].
In Alzheimer's disease, Rnf103 was among six characteristic genes determined by machine-learning algorithms, enabling the prediction of AD progression [3].
In pigs, Rnf103 was considered as a genetic marker for growth traits [5]. In a study on blood lipid levels, Rnf103 was associated with triglycerides [6].
In conclusion, Rnf103 is an important E3 ubiquitin-protein ligase involved in multiple biological processes and diseases. Studies in different models, such as zebrafish for Vibrio infection, have revealed its role in immune-related processes. In diseases like DFU, Alzheimer's, and in relation to lipid metabolism and pig growth, Rnf103 shows potential as a key gene, highlighting its significance in understanding disease mechanisms and potentially developing new treatments.
References：
1. Zheng, Weiwei, Lv, Xing, Tao, Yaqi, Zhu, Tongtong, Xu, Tianjun. 2023. A circRNA therapy based on Rnf103 to inhibit Vibrio anguillarum infection. In Cell reports, 42, 113314. doi:10.1016/j.celrep.2023.113314. https://pubmed.ncbi.nlm.nih.gov/37874674/
2. Yu, Xin, Wu, Zhuo, Zhang, Nan. 2024. Machine learning-driven discovery of novel therapeutic targets in diabetic foot ulcers. In Molecular medicine (Cambridge, Mass.), 30, 215. doi:10.1186/s10020-024-00955-z. https://pubmed.ncbi.nlm.nih.gov/39543487/
3. Lai, Yongxing, Lin, Xueyan, Lin, Chunjin, Chen, Zhihan, Zhang, Li. 2022. Identification of endoplasmic reticulum stress-associated genes and subtypes for prediction of Alzheimer's disease based on interpretable machine learning. In Frontiers in pharmacology, 13, 975774. doi:10.3389/fphar.2022.975774. https://pubmed.ncbi.nlm.nih.gov/36059957/
4. Scheper, Johanna, Oliva, Baldo, Villà-Freixa, Jordi, Thomson, Timothy M. . Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes. In Proteins, 74, 92-103. doi:10.1002/prot.22120. https://pubmed.ncbi.nlm.nih.gov/18615712/
5. Li, Xiaoping, Kim, Sang-Wook, Do, Kyoung-Tag, Choi, Bong-Hwan, Kim, Kwan-Suk. 2010. Analyses of porcine public SNPs in coding-gene regions by re-sequencing and phenotypic association studies. In Molecular biology reports, 38, 3805-20. doi:10.1007/s11033-010-0496-1. https://pubmed.ncbi.nlm.nih.gov/21107721/
6. Li, Changwei, Bazzano, Lydia A L, Rao, Dabeeru C, Lu, Xiangfeng, Kelly, Tanika N. 2015. Genome-wide linkage and positional association analyses identify associations of novel AFF3 and NTM genes with triglycerides: the GenSalt study. In Journal of genetics and genomics = Yi chuan xue bao, 42, 107-17. doi:10.1016/j.jgg.2015.02.003. https://pubmed.ncbi.nlm.nih.gov/25819087/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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