C57BL/6JCya-Cxcr1em1flox/Cya
Common Name:
Cxcr1-flox
Product ID:
S-CKO-07045
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cxcr1-flox
Strain ID
CKOCMP-227288-Cxcr1-B6J-VA
Gene Name
Product ID
S-CKO-07045
Gene Alias
Il8ra
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cxcr1em1flox/Cya mice (Catalog S-CKO-07045) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000053389
NCBI RefSeq
NM_178241
Target Region
Exon 2
Size of Effective Region
~6.1 kb
Detailed Document
Overview of Gene Research
Cxcr1, short for C-X-C chemokine receptor 1, is a key chemokine receptor mainly activated by interleukin 8 (IL-8 or CXCL8). It is expressed in various cells such as leukocytes, fibroblasts, endothelial cells, and smooth muscle cells. Cxcr1, along with Cxcr2, is part of the CXCL8-CXCR1/2 pathways which are essential for the activation and trafficking of inflammatory mediators, and play a role in tumor progression and metastasis [1,2].
Knockout of Cxcr1 inhibits the release of reactive oxygen species (ROS) from neutrophils and synergistically enhances the ability of endothelial cells to eliminate ROS. Cxcr1 knockdown also promotes glutamine metabolism and leads to reduced glutathione by upregulating the expression of malic enzyme 1, which indicates that targeting Cxcr1 has the potential to restore redox homeostasis in hyperoxia-induced lung injury [3].
In conclusion, Cxcr1 is crucial in inflammation-related processes and tumor-associated events. Gene knockout models of Cxcr1 have revealed its role in redox regulation during hyperoxia-induced lung injury, highlighting its potential as a therapeutic target in such disease conditions.
References：
1. Liu, Qian, Li, Anping, Tian, Yijun, Han, Xinwei, Wu, Kongming. 2016. The CXCL8-CXCR1/2 pathways in cancer. In Cytokine & growth factor reviews, 31, 61-71. doi:10.1016/j.cytogfr.2016.08.002. https://pubmed.ncbi.nlm.nih.gov/27578214/
2. Ha, Helen, Debnath, Bikash, Neamati, Nouri. 2017. Role of the CXCL8-CXCR1/2 Axis in Cancer and Inflammatory Diseases. In Theranostics, 7, 1543-1588. doi:10.7150/thno.15625. https://pubmed.ncbi.nlm.nih.gov/28529637/
3. Qin, Hao, Zhuang, Wei, Liu, Xiucheng, Chen, Chang, Zhang, Hao. 2023. Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism. In Cell reports, 42, 112745. doi:10.1016/j.celrep.2023.112745. https://pubmed.ncbi.nlm.nih.gov/37405911/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen